Objective: Evaluation of onset and duration of peripheral nerve blockage with alkalinized lidocaine by borate buffer instead of sodium bicarbonate. Study design: Experimental randomized cross-over study. Animals: Forty male Sprague-Dawley rats (body weight 200–300 g) were used in this study. Methods: Four 1.0% lidocaine solutions were prepared from commercially available solutions. The treat...

A tris-borate buffer solution of = 0.1 and pH 8.9 is shown to be satisfactory for use in the elect-rophoresis of human and bovine sera by the moving-boundary technic. Comparison of the patterns obtained by the use of tris-borate and barbital buffer is presented. Similar numbers of electrophoretically distinct components are found by the use of either buffer. Although the calculated mobility of ...

Native polyacrylamide gel electrophoresis is a powerful approach for visualizing RNA folding states and folding intermediates. Tris-borate has a high-buffering capacity and is therefore widely used in electrophoresis-based investigations of RNA structure and folding. However, the effectiveness of Tris-borate as a counterion for RNA has not been systematically investigated. In a recirculated Hep...

A procedure for obtaining pure glycoasparagine specimens was developed. This method constitutes ion-exchange chromatography in a DEAE-Sephadex A-25 (borate form) column, using a linear gradient of borate buffer as the eluent. With no conversion, almost all the individual glycoasparagines, including some new ones, derived from ovalbumin could be isolated in pure form. By the borate-ion exchange ...

The pH and buffer capacity of dry wood flakes from eleven southern species and their effects on gel time of phenol formaldehyde (PF) oriented strandboard resin with and without zinc borate addition were investigated in this study. It was shown that the pH of the hardwood flakes was acidic, with white oak being the most acidic (pH = 4.60) and elm nearly neutral (pH = 6.93). Southern pine flakes ...

Aldol addition reactions of dihydroxyacetone (DHA) to N-Cbz-amino aldehydes catalyzed by L-rhamnulose-1-phosphate aldolase (RhuA) in the presence of borate buffer are reported. High yields of aldol adduct (e.g. 70-90%) were achieved with excellent (>98 : 2 syn/anti) stereoselectivity for most S or R configured acceptors, which compares favorably to the reactions performed with DHAP. The stereoc...

Four methods of extraction and three methods of concentration of three enteric viruses from mussels were comparatively evaluated by reverse transcriptase PCR (RT-PCR). Shellfish were experimentally contaminated by immersion in seawater seeded with astrovirus, hepatitis A virus, or poliovirus. Sixty-gram samples of mussel tissues were processed by using borate buffer, glycine solution, saline be...

A method, using capillary electrophoresis (CE) with laser-induced fluorescence (LIF) detection for analyzing chitin oligosaccharides is described. Chitin oligosaccharides were derivatized with 9-aminopyrene-1,4,6-trisulfonate (APTS) via reductive amination at 37 degrees C for 16 h (optimized conditions). The APTS-chitin oligosaccharides were analyzed using either an acidic citric acid-phosphate...

Conditions of Electrophoresis.-The apparatus used was similar to that described by Franglen (1953). It consisted of a flat shallow " perspex " box in which a sheet of Whatman No. 1 paper was stretched horizontally with the ends dipping into the electrode solutions. The paper was first wetted with electrolyte, blotted, and placed in position in the apparatus. Serum (10lI.) was then applied from ...

When living cells of Nitella are first exposed to (1) phosphate buffer mixture, or (2) phosphoric acid, or (3) hydrochloric acid, or (4) sodium chloride, or (5) sodium borate, and are then placed in a solution of brilliant cresyl blue made up with a borate buffer mixture at pH 7.85, the rate of penetration of the dye into the vacuole is decreased as compared with the rate in the case of cells t...