The centrifugal liquid membrane (CLM) method, designed for the rapid sample injection, was applied to the kinetic study of the complexation of palladium(II) with 2-(5-bromo-2-pyridylazo)-5-diethyaminophenol (5-Br-PADAP) in the heptane/water system. The formation rates of Pd(II)-5-Br-PADAP complex, which existed only at the heptane/water interface, could be directly measured by the CLM method co...

This paper discusses the development of and proposed enhancements to a colorimetric test for the detection of uranium in biological samples such as urine. The goal of this work is to develop a technique for the detection of uranium that could: 1) be conducted rapidly and accurately; 2) would not require extensive sample preparation; 3) would not require expensive or complicated instrumentation;...

A new method for the simultaneous separation and determination of mercury(II) with other heavy and transition metals by ion chromatography (IC) was developed. This method involves on-column derivatization with 2,6-pyridinedicarboxylic acid (PDCA) and anion-exchange separation, followed by a post-column reaction with 2-[(5-bromo-2-pyridyl)-azo]-5-diethylaminophenol (5-Br-PADAP) to form metal–5-B...

Of two sensitive complexometric reagents for the colorimetry of serum zinc that we investigated, one, 2-(5-bromo-2-pyridylazo)-5-diethylaminophenol (Br-PADAP), was found to be a potentially useful compound for trace-metal determinations. It has a high molar absorptivity (120 000 L mol-1 cm-1) but is not convenient to use because it is not very soluble in water. The other reagent, a related pyri...

a simple, selective and efficient solid phase extraction method based on the use of  γ-alumina nanoparticles coated with sodium dodecyl sulfate (sds) and modified with 2-​(5-​bromo-​2-​pyridylazo)​-​5-​(diethylamino) ​phenol (5-br-padap) as a new adsorbent was developed for the separation and preconcentration of trace amounts of manganese (ii) prior to determination by flame atomic absorption s...

A simple, sensitive and rapid derivative spectrophotometric method using 2-(5-bromo-2-pyridylazo)-5-diethylaminophenol (5-Br-PADAP) has been developed for simultaneous determination of Co(II), Ni(II) and Fe(II) which have very similar chemical behavior and appear together in many real samples. The complexes of all these metal ions with 5-Br-PADAP were formed immediately at pH 7.0 ammonium aceta...

Serratia entomophila and Serratia proteamaculans cause amber disease in the grass grub Costelytra zealandica (Coleoptera: Scarabaeidae), an important pasture pest in New Zealand. Larval disease symptoms include cessation of feeding, clearance of the gut, amber coloration, and eventual death. A 115-kb plasmid, pADAP, identified in S. entomophila is required for disease causation and, when introd...