Optimum conditions for the assay of thiamin were studied using a cyanogen bromide (BrCN) oxidation method. The adopted procedure included neither pre-purification of samples through an ion exchanger nor extraction of the thiochrome into an organic solvent. The 0.25 M BrCN (the concentration before the addition of alkali) and the final NaOH concentration of approx. 1% gave the highest yield of t...

A rare case of bilateral renal cortical necrosis (BRCN) diagnosed only by the characteristic and specific findings of a contrast-enhanced CT scan during the acute initial phase of the disease is presented in this paper. Furthermore, twenty-eight patients of BRCN in the world literatures in English after 1980 were analyzed to investigate the changes in its clinical features over the past 15 year...

The amino-acid composition and molecular weight of actinocarcin, and C-terminal structure of actinocarcin are reported in this paper. By STEIN-MOORE'S and ultraviolet-absorption methods, the molar ratio of amino acids in actinocarcin was determined. The N-terminal amino acids are alanine and valine. The C-terminal amino acid, determined by hydrazinolysis, is tryptophan. The molecular weight is ...

Both cyanogen bromide (BrCN) and 1-ethyl-3-(3'-dimethylaminopropyl) carbodiimide may be used as coupling reagents for the template-directed assembly of DNA duplexes containing the sugar-phosphate backbone modification. Both reagents show similar ligation site structure-specific trend. Practical recommendations are given for selection of the condensing reagent depending on the properties of the ...

The subunit size of thiogalactoside transacetylase from Escherichia coli ML 308 in 6 M guanidinium chloride measured by sedimentation equilibrium in the ultracentrifuge was found to be 29,700 =t 2,000, about half the molecular weight of the native protein. The two chains were identical by estimation of the number of peptides obtained after treatment with trypsin, BrCN, and with both reagents in...

When preparations of rat liver receptor for asialoglycoproteins (rat hepatic lectin, (RHL] are examined by dodecyl sulfate-polyacrylamide gel electrophoresis, multiple polypeptide species are found to be present. The predominant polypeptide has an apparent molecular weight of 41,500 (RHL-1), while two less abundant species appear to be of higher molecular weight (49,000 (RHL-2) and 54,000 (RHL-...

Low-energy dissociative electron attachment to BrCN and CBrCl3 over the temperature range 300–450 K is examined by measuring the velocity and angular distributions of negative ions produced through electron transfer in collisions with velocity-selected K(np) Rydberg atoms. The data are analyzed using a Monte Carlo collision code that models the detailed reaction dynamics. Measurements with BrCN...

von Braun Cyanogen Bromide Reaction, Solasodine, 13C NMR Solasodine on reaction with BrCN yielded two isomeric cyanamides in a ratio of 3:1, which have been characterised as C-22 configurational isomers, N-cyanosolasodine (22 R, 25 R) I IA and N-cyanosolasodine (22 S, 25 Rj II B on the basis of HI and *3C NMR studies. The same reaction with piperidine, yielded its cyanamide, the 13C NMR of whic...

High potential iron-sulfur protein isolated from both autotrophically and heterotrophically grown cells of Chromatium vinosum strain D was found to contain a single 85-amino acid residue polypeptide chain which is covalently linked to the iron-sulfur cluster via 4 residues of cysteine. The molecular weight of the protein, including the 4 atoms of iron and acid labile sulfur, is 9,254. The iron-...