نتایج جستجو برای: CRISPR/Cas
تعداد نتایج: 7 فیلتر نتایج به سال:
1. Jinek, M. et al. Science 337, 816–821 (2012). 2. Cho, S.W., Kim, S., Kim, J.M. & Kim, J.S. Nat. Biotechnol. 31, 230–232 (2013). 3. Cong, L. et al. Science 339, 819–823 (2013). 4. Mali, P. et al. Science 339, 823–826 (2013). 5. Hwang, W.Y. et al. Nat. Biotechnol. 31, 227–229 (2013). 6. Jiang, W., Bikard, D., Cox, D., Zhang, F. & Marraffini, L.A. Nat. Biotechnol. 31, 233–239 (2013). 7. Wang, H...
CRISPR-Cas (clustered regularly interspaced short palindromic repeats—CRISPR associated proteins) is a RNA-guided defense immune system that prevents some genetic elements such as plasmids and virus from getting into the bacterial cells. Zymomonas mobilis is an ethanologenic bacterium, which encodes a subtype I-F CRISPR-Cas system containing three CRISPR loci and a far distant cas gene cluster....
Targeted genome engineering has been instrumental for the study of biological processes, and it holds great promise for the treatment of disease. Historically, gene targeting by homologous recombination has been the preferred method to modify specific genes in mouse and human cells (Fig. 1). However, this approach is hampered by low efficiency, the requirement for drug selection to detect targe...
Most new technologies for manipulating gene expression in mammalian cells are accepted at a relatively slow pace. Occasionally, however, a new technology is so robust and fills such a critical niche that its adoption is widespread and rapid. Fifteen years ago, duplex RNAs were such a technology. RNA interference (RNAi) in mammalian cells was first demonstrated in 2001 (1) and within 2 y RNAi wa...
Cas9 protein of the Type II CRISPRCas (Clustered Regularly Interspaced Short Palindromic Repeats-CRISPR associated) bacterial adaptive immune system emerged recently as a promising tool for genome editing in human and other eukaryotic cells. Cas9 binds a dual crRNA (CRISPR RNA)-tracrRNA (transactivating RNA) molecule, or an artificial single-guide RNA (sgRNA) into a functional complex that acts...
The struggle for life has been the main driving force in natural evolution. Billions of years of competition and warfare between viruses, prokaryotes and eukaryotes (in any combination) have shaped the present world. Among these violent encounters, the attack of bacteria by viruses is probably the most frequently occurring host–parasite interaction on our planet. The offensive bacteriophages ar...
The prokaryotic immune system CRISPRCas is a defence system that protects prokaryotes against foreign DNA. The short CRISPR RNAs (crRNAs) are central components of this immune system. In CRISPR-Cas systems type I and III crRNAs are generated by the endonuclease Cas6. We developed a Cas6b-independent crRNA maturation pathway for the Haloferax type I-B system in vivo, that expresses a functional ...
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