Biologics manufacturing requires the clearance of Host Cell Proteins (HCPs) from recombinant therapeutic protein to acceptable low levels to ensure product purity and patient safety. To ensure adequate removal, a highly sensitive method, commonly in the form of Enzyme-Linked Immunosorbent Assay (ELISA), is necessary to quantify the HCPs amount in process intermediates and drug substance. We rep...

This paper described a convenient semiquantitative method for colorimetric detection of protein with self-calibration integrated on the test strip. Hydrophilic paper was employed as microfluidic device for running colorimetric assay, tree-shaped design was developed to ensure uniform microfluidic flow for multiple branches. The approach was validated with bovine serum albumin (BSA) colorimetric...

A rapid, sensitive and cost-effective method was developed for detection of foodborne pathogens, particularly Salmonella species. The method utilizes single stranded DNA (ssDNA) probes and non-functionalized gold nanoparticles to provide a colorimetric assay for the detection of PCR amplified DNA. Different food samples were tested with the PCR-based colorimetric assay parallel with the convent...

a colorimetric method for detection and assay of mustard gas in urine after addition of thymolphthalein is reported. the detection limit was 80 ng/ml of llrine. mustard gas could also be detected in urine after extraction with gas chromatography using fid detector. the detection limit was 10 ng/ml. in addition, thiodiglycol, a metabolite of mustard gas, could be converted to mustard with concen...

A colorimetric method for detection and assay of mustard gas in urine after addition of thymolphthalein is reported. The detection limit was 80 ng/ml of llrine. Mustard gas could also be detected in urine after extraction with gas chromatography using FID detector. The detection limit was 10 ng/ml. In addition, thiodiglycol, a metabolite of mustard gas, could be converted to mustard with ...

Gold nanoparticles functionalized with thiolated oligonucleotides (Au-nanoprobes) have been used in a range of applications for the detection of bioanalytes of interest, from ions to proteins and DNA targets. These detection strategies are based on the unique optical properties of gold nanoparticles, in particular, the intense color that is subject to modulation by modification of the medium di...

"Naked eye" color detection of proteins was achieved by embedding calixarene receptors within vesicles comprising phospholipids and the chromatic polymer polydiacetylene. Dramatic visible absorbance changes were induced through electrostatic interactions between the protein surface and the vesicle-incorporated hosts. The colorimetric responses could be induced by micromolar protein concentratio...

In order to find a fast, high efficient determination method of Organophosphorus Pesticides (OPPs) residue because OPPs widely used in crops pest control fields in China are causing fearful risks for environment as well as animals and human health, traditional and advanced determination methods were discussed in the study. Based on the spectrum analysis technology combined colorimetric OPPs res...