We investigated the mechanism of suppression of inducible nitric oxide synthase (iNOS) by two beta-carboline alkaloids isolated from Melia azedarach, 4,8-dimethoxy-1-vinyl-beta-carboline (compound 1, C-1) and 4-methoxy-1-vinyl-beta-carboline (compound 2, C-2). iNOS activity in a cell-free extract of lipopolysaccharide/interferon-gamma-stimulated RAW 264.7 cells was found to be markedly increase...

Objective(s): TLR-4 activates a number of inflammatory signaling pathways. Also, AMPK could be involved in anti-inflammatory signaling. The aim of this study was to identify whether stimulation of AMPK could inhibit LPS-induced Tlr-4 gene expression in mice hearts. Materials and methods: Heart AMPK activity and/or Tlr-4 expression was stimulated in different mice groups, using respectively IP i...

The effect of two synthetic lipid A partial structures, compound 406 (or LA-14-PP, identical in structure to the lipid A precursor, known as Ia or IVa) and compound 401 (lipid X), on the in vitro modulation of endotoxin (lipopolysaccharide)-induced interleukin-6 production by human blood mononuclear cells was investigated. Lipopolysaccharide of Salmonella abortus equi and synthetic Escherichia ...

the inhibition of the inducible nitric oxide synthase (inos), cyclooxygenase 2 (cox-2) and nuclear factor-κb (nf-κb) production are research targets of attract in the field of anti-inflammatory drug development. therefore, this study was designed to investigate the anti-inflammatory effects of novel thiazolidinone derivatives using a cellular model of lipopolysaccharide (lps)-stimulated murine ...

Investigation of the effects prantchimgin (Prn) compound isolated from root part Ferulago pauciradiata (Apiaceae) plant in lipopolysaccharide (LPS) cytotoxicity model created L929 cell line. lines were applied a 5% CO2 incubator at 37 °C, using standard culture procedures, LPS was with Prn pure substance final concentration 2 µL and 1 after hour. Following application, MTT protocol, colorimetri...

Background: This study deals with the induction of lipopolysaccharide induced inflammation in animal model and treating this condition using phytomarine R-L compound (Bloomin’Age, Science Living, Milan Italy) that is known to possess anti-inflammatory properties eventually determine their characteristics through in-vivo experimentation.Objective study:The present was subjected test therapeutic ...

To investigate the role of gallic acid in lipopolysaccharide/interferon-gamma induced macrophage polarization toward M1. Macrophages were isolated from peritoneal wash fluid and exposed to prepared at concentrations control, 6.25, 12.5, 25 37.5 μg/ml, presence lipopolysaccharide interferon-gamma, for establishment M1 polarization. Adenosine 5’-monophosphate-activated protein kinase inhibitor co...