AIM The present study was aimed to study the use of cell counts as an early indicator of mammary health. MATERIALS AND METHODS Milk and blood cell counts were estimated from 8 healthy, 8 subclinical (SCM), and 8 clinically mastitis (CM) groups of Karan Fries (KF) cows. RESULTS Total leucocyte counts and neutrophil percent in blood and milk somatic cells and milk neutrophil percent of health...

This study was designed to evaluate serum and milk amyloid A (SAA) as an inflammatory indicator in inflammatory diseases. Twenty clinically healthy cows and 100 cows with various inflammatory diseases were selected for this study. Blood samples were collected from the coccygeal vein of both healthy and diseased groups. Milk samples were taken from four quarters of both groups. Milk samples of f...

Eighteen multiparous early lactation Holstein cows were assigned to a replicated 3 × 3 Latin square with 28-d periods to evaluate milk protein production potential when corn silage (CS) or small grain silage (SGS) was added to diets based on alfalfa silage (AS). Treatments consisted of all AS, a mixture of AS and CS, or a mixture of AS and SGS. Treatment diets were isonitrogenous, isofibrous, a...

Extensive studies have shown the polymorphonuclear leukocytes (PMN) dysfunction inextricably links to parturition. To investigate the effect of parity on PMN function, phorbol 12-myristate 13-acetate (PMA) stimulated luminol-amplified chemiluminescence (CL) and viability of blood and milk PMN were investigated in primiparous and pluriparous dairy cows during periparturient period. The CL kineti...

Cows with superior genetic merit, based on an economic index for milk, fat, and yields (milk-fat-protein dollars), have been designated as elite by USDA. Because of the concern that adjustment for heterogeneous variance may have resulted in inequitable state representation for elite cows, the percentages of US cows with elite status were compared by state using evaluations of registered Holstei...

This study compared the effects of two levels of crude protein (CP) fed during late gestation on the performance, blood metabolites, and ovarian activity of Holstein cows. One-hundred and six cows (42 primigravid and 64 multiparous) 32 d before calving were divided into two groups and fed diets containing moderate (12.7% CP, 36% rumen undegradable protein, (RUP) or high (14.7% CP, 40% RUP) prot...

Polymorphonuclear neutrophil (PMN) function changes during mastitis. To investigate the contribution of milk PMN to the severity of Escherichia coli (E. coli) mastitis, chemiluminescence (CL) of blood and milk PMN and their efficiency to destroy coliform bacteria in the mammary gland were examined following the induction of E. coli mastitis in early lactating cows. To better assess and define t...

The objective of this study was to evaluate the associations among milk production, rectal temperature, and pregnancy maintenance in lactating recipient dairy cows. Data were collected during an 11-mo period from 463 Holstein cows (203 primiparous and 260 multiparous) assigned to a fixed-time embryo transfer (ET) protocol. Only cows detected with a visible corpus luteum immediately prior to ET ...

Cows' milk protein enteropathy is recognised as a significant cause of persistent diarrhoea and malabsorption in young infants, but there are as yet no generally accepted diagnostic criteria. A combined clinical and histological approach to the diagnosis of cows' milk protein-sensitive enteropathy has been used in 15 patients, and the following set of criteria are proposed. (1) Clinical disease...