A simple and sensitive method for the determination of cynarin is described and partially validated. The assay was used to determine the pharmacokinetic profile of cynarin after Wistar rat intravenous administration. Cynarin was isolated from rat plasma by solid-phase extraction. Separations were performed by reversed-phase high-performance liquid chromatography with ultraviolet detection. RESU...

Artichoke is used to mobilize fatty stores in the liver and detoxify it, and as a natural aid to lower cholesterol. Cynarin demonstrated properties against oxidative stress induced by inflammatory mediators. In addition it exercises a certain control on lipolysis, favouring the synthesis energy-transporting enzymes NAD-NADH2 and NADP-NADPH2. It acts as diuretic and vasoconstrictor, stimulating ...

Artichoke (Cynara scolymus L.) is one of the world's oldest medicinal plants with multiple health benefits. We have previously shown that artichoke leaf extracts and artichoke flavonoids upregulate the gene expression of endothelial-type nitric oxide synthase (eNOS) in human endothelial cells. Whereas NO produced by the eNOS is a vasoprotective molecule, NO derived from the inducible iNOS plays...

The antioxidant activity and total phenolic content of Morinda tinctoria leaves was evaluated. The successively extracted leaves of Morinda tinctoria using various solvents was analyzed for their total phenolic content. The extracts were subjected to column chromatography for the isolation of bioactive molecules. In vitro antioxidant activity was evaluated by employing different assays, includi...

An efficient method for the rapid separation and purification of polyphenols from artichoke by polyamide column chromatography in combination with high-speed counter-current chromatography (HSCCC) was successfully built. The crude ethanol extracts from dry artichoke were first pre-separated by polyamide column chromatography and divided in two parts as sample 1 and sample 2. Then, the samples w...

Chelation, electrochemical, antioxidant and cytoprotective properties of six phenolics - cynarin and caffeic, chlorogenic, ferulic, protocatechuic and rosmarinic acids were studied on the following models: (i) chelation of transition metals, (ii) quenching of the diphenylpicrylhydrazyl radical (DPPH), (iii) determination of half-wave potential, (iv) erythrocytes or mitochondrial membranes damag...