the ovine melatonin receptor 1a (mtnr1a) and aromatase (cyp19) genes were structurally characterized and the association between their variants and reproductive and growth traits was studied in kurdi sheep at kurdi sheep breeding station located in shirvan, iran. the genomic dna was extracted by guanidine thiocyanate-silica gel method. polymerase chain reaction was carried out to amplify 824 bp...

Cytochrome P450 aromatase is the key enzyme in estrogen biosynthesis, encoded by CYP19 gene. Impairment of spermatogenesis associated with a decrease in sperm motility and inability to fertilize oocytes in mice due to the lacking of CYP19 was observed. However, it is little known about the CYP19 roles in boar spermatogenesis and fertility. Therefore, the aim of this research was to investigate ...

The irreversible transformation of androgens into oestrogens is catalysed by cytochrome P450 aromatase. In the present study, we explored the contribution of the (TTTA)(n) polymorphism in the aromatase gene (CYP19) to sperm concentration and motility. Ninety normozoospermic and 60 oligospermic men were examined during infertility examinations. DNA was extracted from spermatozoa, and the CYP19 (...

The relationship between the SNP of the cytochrome P450 gene (CYP19-PvuII) and milk production traits of Black-and-White and Jersey cattle were analysed. A total of 437 cows were included in the study. A PCR-RFLP was used to genotype. The frequencies of genotypes and alleles for the Black-and-White cows were as follows: 0.8985 AA, 0.0977 AB, 0.0038 BB, and 0.9474 CYP19, 0.0526 CYP19. In the Jer...

BACKGROUND AND OBJECTIVES Testicular Leydig cell tumours (LCTs) are rare, steroid-secreting tumours. Elevated levels of aromatase (CYP19 or CYP19A1) mRNA have been previously described in LCTs; however, little is known about the mechanism(s) causing CYP19 over-expression. We report an LCT in a 29-year-old male with elevated plasma oestradiol caused by enhanced CYP19 transcription. DESIGN AND ...

OBJECTIVE Experimental evidence suggests that fetal exposure to androgen excess may program the development of polycystic ovary syndrome (PCOS) in utero. The aim of this study was to examine whether the sex hormone binding globulin (SHBG)(TAAAA)n and the cytochrome P450, family 19 (CYP19)(TTTA)n polymorphisms, known to influence sex hormone-binding globulin (SHBG) levels and aromatase activity ...

요 약 본 연구는 생후 발달과정에서 숫 흰쥐의 생식기인 Efferent ductules (EDs)과 부정소에서 cytochrome P450 aromatase (Cyp19) 유전자의 발현과 연령별 발현 양상을 알아보는데 목적이 있다. 조직으로부터 추출된 total RNA는 역 전사 반응을 통해 cDNA로 바뀌어진 후 real-time PCR 기법을 사용하여 부위 별 그리고 연령별로 Cyp19 유전자 발현을 알아보았다. Cyp19 유전자의 발현은 EDs에서 90일령을 제 외한 7일, 14일, 그리고 30일령 등 성숙기 이전의 모든 연령에서 나타났으며, 부정소에서는 7일령을 제외한 모든 연령에서 보여졌다. 특히 부정소에서 Cyp19의 발현은 부정소의 각 부위별로 특이한 양 상을 보였다. 본 연구 결과를 통해...

OBJECTIVE Extragonadal estrogen biosynthesis is relevant for the regulation of bone metabolism. The aims of this paper were: (i) to examine CYP19 (aromatase) gene expression in primary cultures of osteoblasts under several hormone and cytokine treatments and (ii) to study the promoter usage of CYP19 in these cells. METHODS Primary cultures of osteoblasts were obtained from healthy donors. The...

In humans, aromatase (CYP19) gene expression is regulated via alternative promoters. Activation of each promoter gives rise to a CYP19 mRNA species with a unique 5'-untranslated region. Inhibition of aromatase has been reported to downregulate lung tumor growth. The genetic basis for CYP19 gene expression and aromatase activity in lung cancer remains poorly understood. We analyzed tissues from ...

The aromatase-Cre recombinase (Cyp19-Cre) transgenic mouse model has been extensively used for placenta-specific gene inactivation. In a pilot study, we observed unexpected phenotypes using this strain, which prompted an extensive characterization of Cyp19-Cre placental ROSAmT/mG reporter mice. two strains were mated to generate bi-transgenic Cyp19-Cre;ROSAmT/mG mice following standard breeding...