نتایج جستجو برای: DNA Molecular weight marker

تعداد نتایج: 1460852  

Fatemeh Moradian, Hamed Hekmatnezhad Seyed Hamidreza Hashemi-Petroudi

DNA size markers (ladder) are essential tools in molecular biology, genetics and biotechnology. In this study, a simple and cost-effective method for laboratory production of DNA ladders is introduced. For this purpose, different sizes of 100 to 2000 bp DNA segments were designed using PCR technique. For producing 14 different gene fragments as DNA molecular weight markers, recombinant plasmid ...

Journal: :iranian journal of microbiology 0
mohammad sekhavati department of veterinary aerobic bacteria, razi institute, karaj, iran. keyvan tadayon department of veterinary aerobic bacteria, razi institute, karaj, iran. rainak ghaderi department of veterinary aerobic bacteria, razi institute, karaj, iran. reza banihashemi department of veterinary aerobic bacteria, razi institute, karaj, iran. ahmadreza jabbari department of veterinary aerobic bacteria, razi institute, karaj, iran. gholamreza shokri department of veterinary aerobic bacteria, razi institute, karaj, iran.

background and objectives : dna molecular weight marker is widely used in molecular biology experiments incurring considerable costs on low-budget settings. materials and methods : here a pcr-supported procedure is described that uses 10 primer pairs targeting chromosomal dna from the harmless vaccinal bacillus anthracis sterne 34f2 strain as template. a single pcr protocol is used to reproduce...

Journal: :Genetics and molecular research : GMR 2011
T-Y Wang L Wang F Wang

Serving as a DNA molecular weight standard, the DNA ladder has been widely used in molecular biology applications. We developed a simple method for the preparation of a DNA marker, which involves designing primers to amplify 100- to 1000-bp DNA fragments using lambda DNA as a template for polymerase chain reaction, followed by extraction with phenol/chloroform, precipitation with ethanol and mi...

2010
Tian-Yun Wang Li Guo Jun-he Zhang

DNA molecular weight standard control, also called DNA marker (ladder), has been widely used in the experiments of molecular biology. In the paper, we report a method by which DNA marker was prepared based on multiple PCR technique. 100-1000 bp DNA fragments were amplified using the primers designed according to the 6631 ~ 7630 position of lambda DNA. Target DNA fragments were amplified using T...

2015
Mohammad Sekhavati Keyvan Tadayon Rainak Ghaderi Reza Banihashemi Ahmad Reza Jabbari Gholamreza Shokri Nasim Karimnasab

BACKGROUND AND OBJECTIVES DNA molecular weight marker is widely used in molecular biology experiments incurring considerable costs on low-budget settings. MATERIALS AND METHODS Here a PCR-supported procedure is described that uses 10 primer pairs targeting chromosomal DNA from the harmless vaccinal Bacillus anthracis Sterne 34F2 strain as template. A single PCR protocol is used to reproduce a...

Azita Zadeh-Vakili, Gh. Rastegar Lari P. Eshghi

Background: Indirect genetic diagnosis using polymorphic DNA markers can detect carriers of hemophilia A. This technique is preferable in developing countries because of its simplicity and cost effectiveness compared to direct mutation analysis. In the present study, we examined usefulness of intragenic marker BclI restriction fragment length polymorphism (RFLP) at intron 18, for carrier detect...

احمد شهرضا, حسین خان, بهدانی, مهدی, ریخته‌گران تهرانی, زهرا, سعیدی جم, مسعود, شباب, نوشین, کریمی زارع, سکینه,

Background: Molecular DNA markers are one of the most important tools in molecular biology labs. The size of DNA molecules is determined by comparing them with known bands of markers during gel electrophoresis. There are many different protocols to produce these kinds of molecular markers. In this study we have suggested an efficient strategy to produce molecular weight markers in industrial pr...

Journal: :iranian journal of medical sciences 0
azita zadeh-vakili p. eshghi gh. rastegar lari

background: indirect genetic diagnosis using polymorphic dna markers can detect carriers of hemophilia a. this technique is preferable in developing countries because of its simplicity and cost effectiveness compared to direct mutation analysis. in the present study, we examined usefulness of intragenic marker bcl i restriction fragment length polymorphism (rflp) at intron 18, for carrier detec...

Journal: :mycologia iranica 0
s. ebrahimi department of plant protection, faculty of agriculture, university of zabol, zabol, iran s. k. sabbagh department of plant protection and institute of plant biotechnology, university of zabol, zabol, iran m. aminaei research center of agriculture and natural resources of kerman province, kerman, iran f. khosravimoghadam department of plant protection, faculty of agriculture, university of zabol, zabol, iran f. rostami department of plant protection, faculty of agriculture, university of zabol, zabol, iran

die-back disease caused by paecilomyces variotti is one of the most destructive diseases of pistachio in iran. in this study, genetic diversity of paecilomyces variotti isolates was investigated with ssr marker, using six primers pairs. sampling was done from pistachio gardens in different geographical zones of kerman province during summer 2013. various fungal isolates were isolated from infec...

Members the DNA Methyltransferases (DNMT) gene family have been shown to play fundamental roles in regulating embryonic growth and development from embryonic fertilization to postnatal life; through regulating the establishment and/or maintenance of specific epigenetic marks. The present study was conducted to identify potential reported mutations within the exon 33 of DNMT-1, intron 4 of DNMT-...

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