The effective delivery of protein therapeutics remains a major challenge. Here, the novel nanoplatforms with bovine serum albumin (BSA)-coated, Dnase-1-loaded MnOx nanoflowers are developed (denominated MD@BSA). These MD@BSA present highly efficient loading and Dnase-I into tumor cells. Under glutathione (GSH) overexpression environment, is degradable, resulting in Dnase-1 Mn2+ release for comb...

Dermatophytes are keratinophilic fungi and the causative agent of dermatophytosis in animals people. In pathogenesis this disease, enzymes such as DNase, gelatinase, lipase, keratinase, elastase, collagenase highlighted. This work aimed to verify production these by clinical environmental isolates dermatophytes. Environmental strains were obtained Vanbreuseghem technique (1952), using soil samp...

Among DNase I family members, only DNase gamma causes DNA fragmentation during apoptosis. However, the molecular basis for this functional feature of DNase gamma is poorly understood. Here we describe the identification of functional NLSs (nuclear localization signals) in DNase gamma and their roles in its apoptotic function. DNase gamma contains two NLSs: a classical bipartite-type NLS (NLS1) ...

The cornified layer, the stratum corneum, of the epidermis is an efficient barrier to the passage of genetic material, i.e. nucleic acids. It contains enzymes that degrade RNA and DNA which originate from either the living part of the epidermis or from infectious agents of the environment. However, the molecular identities of these nucleases are only incompletely known at present. Here we perfo...

The serum of patients with many autoimmune (AI) diseases contains small fractions of antibodies possessing both DNase and RNase activities. It was shown that immunization of rabbits with DNA, RNA, DNase I and RNase leads to production of antibodies with DNase and RNase activities. It is not known whether anti-idiotypic antibodies against DNase II can possess DNase or RNase activity. Electrophor...

Recombinant human deoxyribonuclease I (DNase I) is an important clinical agent that is inhaled into the airways where it degrades DNA to lower molecular weight fragments, thus reducing the viscoelasticity of sputum and improving the lung function of cystic fibrosis patients. To investigate DNases with potentially improved properties, we constructed a molecular fusion of human DNase I with the h...

DNase X is the first human DNase protein identified as being homologous with DNase I. In the present study we describe the isolation of several mammalian DNase X cDNAs and the molecular characterization of their coding proteins. A sequence comparison reveals some conserved characteristics: all the mammalian DNase X proteins have an N-terminal signal peptide, a potential N-linked glycosylation s...

A study was undertaken to determine the subcellular location of DNase-I, a major acid deoxyribonuclease in Drosophila melanogaster. Embryonic tissue used in these experiments was derived from a wild type strain and from a strain homozygous at the DNase-l locus (3-61.8) for the null activity allele, DNase-lnl24. The majority of total acid DNase and DNase-l activity is found in the small particul...