there are many techniques to knock out directed genes in bacteria, some of which have been described in salmonella species. in this study, a combination of soeing pcr method and the λ red disruption system were used to disrupt phop gene in wild type and standard strains of salmonella typhimurium. three standards pcr and one fusion pcr reactions were performed to construct a linear dna including...

with the introduction of the use of seatbelts in cars, mortality following motor vehicle crashes has decreased significantly. however, two patterns of injuries, the ‘seatbelt sign’ and ‘seatbelt syndrome’ have emerged. injuries may consist of traumatic abdominal wall disruption. we present two cases of severe abdominal wall disruption caused by a seatbelt injury and treated with primary repair....

costochondral and rib grafts have long been used for reconstruction of lost condyle. donor site morbidity has less been under investigation and researched. pleural disruption pneumothorax and ugly scars are among the donor site complications. this study aims to determine the rate of pleural disruption following costochondral graft harvesting from the 4th to the 7th ribs of right and left ribs f...

in this study, the effect of irrigation regimes and plant nutrition were evaluated as split plot arrangment based on randomized complete block design with three replications in 2010. treatments consisted of irrigation regimes at three levels: well-irrigation, irrigation disruption at vegetative growth stage, and irrigation disruption at reproductive growth stage as main plots and combination of...

introduction: codling moth, cydia pomonella is one of the key pests of apple in khorasan razavi province which annually causes severe fruit damage to apple crop. there are several ways that are used to control and prevent injury to apple products in the world. the most successful and widespread use of pheromones has been in monitoring traps. mating disruption method by pheromones takes place wh...

ET-07. DOES OSMOTIC BLOOD-BRAIN BARRIER DISRUPTION ALLOWS EFFLUX PUMP SUBSTRATES DELIVERY TO THE

supplier selection is one of the most critical activities of purchasing management in supply chain and managers increasingly face sourcing decisions of how to selected suppliers. this paper illustrates the development of a sourcing decision that provides support for the buyer firm in supply chain.the models developed here, involved selecting between single and dual sourcing. outside and local s...

background: among all common techniques in sitedirectedmutagenesis, λ red recombinase system has beenwidely used to knock out chromosomal genes in bacteria. in thismethod, there is always the risk of dna linear digestion byhost's restriction enzymes that leads to the low frequency ofrecombination. objectives:to overcome this, we constructeda recombinant vector to disrupt phop gene in salmonella...