objective: acute mesenteric ischemia (ima) is a vascular emergency with broad variability of clinical presentations and non-specific laboratory findings. therefore, there is a significant need for reliable serological markers of ischemia. various laboratory studies may be performed for suspected ami, but in general, such studies will not establish the diagnosis. methods: in a prospective, non-i...

Fragment D (M, 100000) prepared from a terminal plasmin digest of fibrinogen was isolated and used to study its effect on fibrin formation. Increasing amounts of fragment D added to a solution of fibrinogen and thrombin decrease the rigidity of the resultant gel (10% of control at 2mol of fragment D/mol of fibrinogen). Half-maximal inhibition is achieved at 1 mol of fragment D/mol of fibrinogen...

Concentrations of cross-linked fibrin degradation products (XL-FDPs) in plasma, measured by enzyme-linked immunosorbent assays (ELISAs) based on monoclonal antibodies (MAbs) raised against fragment D-dimer of cross-linked fibrin, increase when patients are given fibrinolytic agents. Whether XL-FDPs derive from circulating cross-linked fibrin polymers in plasma, compared with clot-associated fib...

The different species of Fragment D were purified from plasmin digests of either fibrinogen or highly cross-linked fibrin and examined electrophoretically in sodium dodecyl sulfate on polyacrylamide gels before and after reduction by ,&mercaptoethanol. It was found that Fragment D from cross-linked fibrin is approximately twice the size of that from fibrinogen, which indicates that the COOH-ter...

The aim of this work is to study the intermolecular interactions fibrin with D-domain-containing fragments fibrin(ogen): D-dimer and D-fragment. Materials methods. Human fibrinogen was obtained from human blood plasma by salt extraction using 16 % Na2SO4. content protein coagulated thrombin – 96-98%. Analytical size-exclusion chromatography for detection molecular complexes performed on Sepharo...

Three Fragment D species (D1, D2, D3) were isolated with time from a plasmin digest of fibrinogen and had molecular weights of 92,999, 86,000 and 82,000 by summation of subunit molecular weights from sodium dodecyl sulfate polyacrylamide gel electrophoresis. Their molecular weights by sedimentation equilibrium ultracentrifugation were 94,000 t87,000, 88,000 to 82, 000, and 76,000 to 70,000 depe...

Plasmic degradation of crosslinked human fibrin produces unique degradation products. (DD)E complex and fragment DD, in which the DD moiety contains two fragment D molecules joined together by covalent bonds. In this study. fragment DD antigenic markers were studied using two different antisera: one against intact (DD)E complex, and the other against fragment DD that has been exposed to 3 M ure...

The mechanism of association and the organization of human fibrin were studied by using affinity chromatography. Insolubilized fibrinogen, fibrin monomer, and crosslinked fibrin were used to localize the binding sites on fibrinogen and fibrin derivatives. Four different polymerization sites have been distinguished. A binding site ("a"), available without thrombin action, is present on the fibri...

Essentials Glycoprotein VI (GPVI) binds collagen, starting thrombogenesis, and fibrin, stabilizing thrombi. GPVI-dimers, not monomers, recognize immobilized fibrinogen and fibrin through their D-domains. Collagen, D-fragment and D-dimer may share a common or proximate binding site(s) on GPVI-dimer. GPVI-dimer-fibrin interaction supports spreading, activation and adhesion involving αIIbβ3. SUM...

The biological effects of the binding of fibrin(ogen) degradation products to M protein-bearing group A streptococci were investigated. Type 24 group A streptococci bind fibrinogen degradation products of the D family, but not fragment E. Binding appears to be mediated by M protein, since a large peptide of this molecule (pep M24) bound to fragments containing the terminal domains of the fibrin...