the present study describes the encapsulation of protocorm of cymbidium bicolor lindl. from 60 days-old in seed cultures for short- term conservation and propagation. various concentrations and combinations of gelling matrix (sodium alginate) and complexing agents (calcium chloride) were tested to prepare uniform beads. the ideal beads were ob- tained through a combination of 3% sodium alginate...

The loss of plant genetic resources has made necessary the development of new ex situ conservation methods. Advances in biotechnology provide new methods for plant germplasm conservation and evaluation. Biotechnological tools like in vitro culture, cryopreservation, and molecular markers offer a valuable alternative to plant diversity studies, management of genetic resources and ultimately cons...

For human food security, the preservation of 7.4 million ex-situ germplasm is a global priority. However, ex-situ-conserved seeds are subject to aging, which reduces their viability and ultimately results in the loss of valuable genetic material over long periods. Recent progress in seed biology and genomics has revealed new opportunities to improve the long-term storage of ex-situ seed germpla...