Background:Pseudomonas aeruginosa is a gram negative non facultative bacterium and one of the members of normal flora in different sites of body in healthy humans.this bacterium can resist in fluids and hospital environments for a long time.Pseudomonas aeruginosa has two systems for glucose uptake:a low affinity oxidative pathway and a high affinity phosohorylative pathway.Although the role of ...

Decreased biomass growth in iron (Fe)-limited Pseudomonas is generally attributed to downregulated expression of Fe-requiring proteins accompanied by an increase in siderophore biosynthesis. Here, we applied a stable isotope-assisted metabolomics approach to explore the underlying carbon metabolism in glucose-grown Pseudomonas putida KT2440. Compared to Fe-replete cells, Fe-limited cells exhibi...

The methyl alpha-glucoside-transport system of Pseudomonas aeruginosa has been characterized with respect to its specificity, energy-dependence, kinetics and regulation. The uptake of glucose involved two components, one of which transported glucose (K(m)=8mum) and methyl alpha-glucoside (K(m)=2.8mm) whereas the other was more complex, involving the extracellular activity of glucose dehydrogena...

Growth of Escherichia coli O157:H7 strain ATCC 43895 was monitored at 5, 10, 15, and 25 degrees C in both pure and mixed (1:1) cultures with a gluconate-producing Pseudomonas sp. found in meat to evaluate the effect of the absence and presence of 1% glucose in broth on temperature-dependent competition. The number of colonies of the Pseudomonas strain exceeded 9 log CFU/ml under all conditions ...

plant growth promoting rhizobacteria and arbuscular mycorrhizal fungi can inhanced plant growth by increasing nutrient uptake. in order to investigate the effect of pgpr and amf on some morphological parameters, macro (nitrogen, phosphorus, potassium, calcium and magnesium) and micro (iron, zinc and copper) nutrients uptake, percentage of root colonization and some morphological factors of pepp...

the current epidemic of diabetes indicates the need of proper and effective medications that are limited in their potency to have many side effects. thus, introduction of alternative and complementary medicine is now in picture. the main objective of this study was to analyze the rate of glucose transport across cell membrane in yeast cells system in the presence of the ethanolic extract of bru...

Evidence is presented that Zn(2+) and certain other multivalent cations inhibit the uptake and oxidation of substrates by Pseudomonas aeruginosa and by other bacterial cells. Tris(hydroxymethyl)aminomethane augmented this effect. Inhibition by these multivalent cations could be prevented by incubation of cellular suspensions of P. aeruginosa in phosphate buffer. In the case of other microorgani...

Diabetes is associated with increased frequency of hospitalization due to bacterial lung infection. We hypothesize that increased airway glucose caused by hyperglycaemia leads to increased bacterial loads. In critical care patients, we observed that respiratory tract bacterial colonisation is significantly more likely when blood glucose is high. We engineered mutants in genes affecting glucose ...