To investigate the dynamic cellular response to a condition change, selective labeling of the nascent proteome is necessary. Here, we report a method combining click chemistry protein labeling with 2D DIGE. To test the relevance of the method, we compared nascent proteomes of actively growing bacterial cells with that of cells exposed to protein synthesis inhibitor, erythromycin. Cells were inc...

The effect of flux on photoluminescence properties Strontium cerium oxide doped Sm3+ phosphor was studied. synthesized by conventional solid state reaction method at temperature 1200°C for 4h. powder samples were characterized X-ray diffraction (XRD), Scanning Electron microscope (SEM) and Fourier transforms infrared spectroscopy (FTIR) spectrum. X-Ray pattern reveals the crystallite size struc...

Die Sortase-vermittelte Ligation (Engl. sortase-mediated ligation, SML) ist ein effektives Werkzeug der Proteinchemie, welches die von Peptiden mit LPxTG-Sortiermotiven und N-terminalen Glycin-Nukleophilen ermöglicht. Kopplung mehrerer Fragmente durch SML wird den erneuten Einbau eines Sortiermotivs in das Produkt verhindert. Hier beschreiben wir Multi-Fragment-SML auf Grundlage schaltbaren Sor...

We measured growth and development variables on single soybean [Glycin max (L.) Merr.] plants under five management strategies in the upper Midwestern USA. Plants grown under different management strategies differed significantly in their geometric structures, and were classified into their proper categories with 75 to 100% correct classification, based mainly on differences in their fractal di...