Identification of 19 molecular species globotriaosylceramides (Gb3) in extracts from a Fabry's plasma patient and healthy control was performed by High-Performance Thin-Layer Chromatography (HPTLC)-densitometry online coupling to Mass Spectrometry (MS). Separation carried out on LiChrospher plates using Automated Multiple Development (AMD). Densitometry twin combining detection the visible at 5...

a simple, accurate and rapid high performance thin layer chromatography (hptlc)-densitometric method was developed for separation and determination of cetirizine (cet) as a long acting antihistamine and montelukast (mon) as an antileukotriene in pharmaceutical dosage forms. the compounds were separated on silica gel 60 f254 hptlc plates using a mixture of ethyl acetate : methanol : ammonia solu...

a simple, accurate and precise hptlc- densitometry method for the simultaneousdetermination of abacavir, lamivudine and zidovudine in pharmaceutical tablet has beendeveloped. separation of tablet components was performed on aluminum backed hptlc plates(silica gel 60 f254 20×20 cm with 0.2 mm thickness, camag, muttenz, switzerland ) usingmethanol : chloroform : acetonitrile (4 : 8 : 3 v/v). good...

This paper describes a convenient and practice method for quantitation of surfactant phospholipids (1,2-dipalmitoyl-3-sn-phosphatidyl choline [DPPC] and 1-palmitayl-2-oleyl-3-sn-phosphatidyl glycerol [POPG]) in a recombinant surfactant lyophile (Venticute) by high-performance, thin-layer chromatography (HPTLC) with video densitometry. DPPC and POPG were extracted from Venticute-lyophile using m...

We have devised a high performance thin-layer chromatography (HPTLC) densitometry method to resolve the major lipid classes of brain tissue. We used DEAE-Sephadex column chromatography to separate the total lipid into neutral and acidic lipid fractions. The lipid fractions were then spotted on separate HPTLC plates and chromatographed in one dimension using two solvent systems. Quantitation was...

PURPOSE In the present study we aimed to quantify marrubiin, as the major active compound, in the aerial parts of Marrubium vulgare from Iran using a HPTLC-densitometry technique. METHODS Quantitative determination of marrubiin in M. vulgare methanol extract was performed by HPTLC analysis via a fully automated TLC scanner. Later on, the in vitro antioxidant activity of the M. vulgare methano...

vitex agnus-castus l. is a medicinal plant which is used in several dosage forms for women hormonal disorders and standardized according to the iridoids or flavonoids content. aucubin, an iridoid glycoside, considered as a marker in some formulations. in this research, a thin layer chromatographic method with densitometric detection has been developed for quantitative determination of aucubin i...

Objective: This study aimed to investigate the anti-inflammatory activity of methanolic leaf extract Houttuynia cordata Thunb. from Mizoram, India, using in-vitro and in-vivo models. In addition, also determine quercetin content high-performance thin-layer chromatography (HPTLC) densitometry. Methods: The H. was prepared successive Soxhlet extraction, its evaluated models such as inhibition pro...

A new, simple, precise, and rapid high-performance thin-layer chromatography (HPTLC) method was developed for the analysis of commercial emulsifiable concentrate (EC) formulations of cypermethrin, alpha-cypermethrin, and lambda-cyhalothrin. A known amount of reference grade synthetic pyrethroid and its EC formulation was subjected to TLC, and the amount of pesticide present in the active ingred...

Soy (Glycine max L., Fabaceae) and soy products are becoming more popular because of their low toxicity and therapeutic effects. Soy possesses antioxidant, anti-inflammatory and anti-allergic properties, however, the most important is its estrogenic activity associated with occurrence of phytoestrogens. Isoflavones with phytoestrogenic effects were determined in four commercially available soya...