Interest has recently been revived in the possibility that urocanic acid may be an intermediate in the metabolism of L-histidine (1). In the rat, both Cox and Rose (2) and Harrow and Sherwin (3) found this product ineffective in the dietary replacement of histidine. However, neither it nor imidazolelactic acid has been tested for its capacity to promote liver glycogen deposition, which would se...

l-Histidine biosynthesis is an ancient metabolic pathway present in bacteria, archaea, lower eukaryotes, and plants. For decades l-histidine biosynthesis has been studied mainly in Escherichia coli and Salmonella typhimurium, revealing fundamental regulatory processes in bacteria. Furthermore, in the last 15 years this pathway has been also investigated intensively in the industrial amino acid-...

Histamine production from histidine in fermented food products by lactic acid bacteria results in food spoilage and is harmful to consumers. We have isolated a histamine-producing lactic acid bacterium, Lactobacillus hilgardii strain IOEB 0006, which could retain or lose the ability to produce histamine depending on culture conditions. The hdcA gene, coding for the histidine decarboxylase of L....

The catalytic histidine of human neutrophil elastase was replaced with alanine (H57A) to determine if a substrate histidine could substitute for the missing catalytic group-'substrate-assisted catalysis'. H57A and wild-type elastase were recovered directly from Pichia pastoris following expression from a synthetic gene lacking the elastase pro sequence, thereby obviating the need for zymogen ac...

Differences in histidine protonation result contrasting dynamics of the PCB chromophore AnPixJg2. The doubly protonated form induces heterogeneity, which is absent singly form. They can be distinguished using CD spectroscopy.

A histidine fermenting Clostridium was isolated in this laboratory by Dr. Arthur Kornberg by standard enrichment culture techniques. This bacterium, which can use either L-histidine or i-glutamic acid as a primary energy source, has been identified as Clostridium tetanomorphum (Breed et al., 1948). Cell suspensions of the organism have been shown to form carbon dioxide, ammonia, hydrogen, forma...

Metal-ligand interactions with various proteins form in vivo metal assemblies. In recent years, metallosupramolecular approaches have been utilized to forge an assortment of fascinating twoand three-dimensional nano-architectures, and macroscopic materials, such as metal-ligand coordination polymeric materials, have promise in artificial systems. However to the best of our knowledge, the self-a...

Pig heart supernatant malate dehydrogenase is alkylated by 250 mM iodoacetamide at 37 degrees C in pH 7.5 Tris/acetate buffer which is 0.05 M with respect to acetate to form enzyme with 1,3-dicarboxamidomethyl histidine, 3-carboxamidomethyl histidine, 1-carboxamidomethyl histidine, carboxamidomethyl cysteine, and carboxamidomethyl methionine. 1,3-Dicarboxamidomethyl histidine forms with a stoic...

Phosphorylation of histone H4 on one or both of its two histidine residues has been known to occur in liver cells for nearly 40 years and has been associated with proliferation of hepatocytes during regeneration of the liver following mechanical damage. More recently, large increases in histone H4 histidine kinase activity have been found to occur associated with proliferation and differentiati...