background: clinical evidence indicates the diabetes-induced impairment of osteogenesis caused by a decrease in osteoblast activity. flavonoids can increase the differentiation and mineralization of osteoblasts in a high-glucose state. however, some flavonoids such as luteolin may have the potential to induce cytotoxicity in osteoblast-like cells. this study was performed to investigate whether...

AIM The insulin-like growth factor 1 (IGF1) gene gives rise to multiple transcripts, using an elaborate alternative splicing mechanism. The aim of this study was to shed light on the expression and role of the IGF1 system in human MG-63 osteoblast-like osteosarcoma cells. MATERIALS AND METHODS The expression of the IGF1Ea, IGF1Eb and IGF1Ec isoforms was characterized using reverse transcripti...

Background: Clinical evidence indicates the diabetes-induced impairment of osteogenesis caused by a decrease in osteoblast activity. Flavonoids can increase the differentiation and mineralization of osteoblasts in a high-glucose state. However, some flavonoids such as luteolin may have the potential to induce cytotoxicity in osteoblast-like cells. This study was performed to investigate whether...

We have previously shown the expression of the extracellular calcium (Ca2+o)-sensing receptor (CaR) in osteoblast-like cell lines, and others have documented its expression in sections of murine, bovine, and rat bone. The existence of the CaR in osteoblasts remains controversial, however, since some studies have failed to document its expression in the same osteoblast-like cell lines. The goals...

conclusions mg-63 and saos-2 osteosarcoma cell lines might not represent appropriate experimental models for studies that aim to analyze signal transduction in osteoblasts. background a large number of phospholipase c (plc) enzymes, both mrna transcripts and proteins, have been detected in osteoblasts, corroborating the importance of calcium regulation in bone tissue. mg-63 and saos-2 human ost...

One-third of women with breast cancer will develop bone metastases and eventually die from disease progression at these sites. Therefore, we analyzed the ability of human MG-63 osteoblast-like cells (MG-63 cells), MG-63 conditioned media (MG-63 CM), insulin-like growth factor I (IGF-I), and transforming growth factor beta 1 (TGF-beta1) to alter the effects of adriamycin on cell cycle and apopto...

In vitro studies evaluating the cytotoxic potential of substances released from dental adhesives are lacking. The purpose this study was to compare cytotoxicity extracts Scotchbond Universal and Optibond Solo Plus, an adhesive in experimental phase: T1. 3T3 mouse fibroblast cells MG-63 osteoblast-like human osteosarcoma were exposed for 24 h serial extract dilutions. Cytotoxicity determined usi...