The AN703 antibody, derived from the 221-42-1 hybridoma, detects PDI-containing endoplasmic reticulum of Dictyostelium discoideum by immunofluorescence.

introduction: binding a monoclonal antibody to tumor associated antigens is an effective method for cancer therapy because these agents can specifically target malignant cells, in fact monoclonal antibodies are effective agents for diagnosis, grading and treatment of different kinds of cancers. methods: in this research, a new monoclonal antibody against colon cancer cells was prepared and anti...

introduction: production of monoclonal antibody against alkaline phosphatase for application in immunohistochemical and immunocytochemical techniques such as alkaline phosphatase anti-alkaline phosphatase (apaap) method. material and methods: in this investigation female balb/c mice were immunized by several injections of alkaline phosphatase and the antibody titer in their sera were measured a...

different igg subclass profiles are produced in response to different antigenic stimuli in a variety of diseases. igg subclass levels may reflect disease severity. quantification of igg subclasses depends on the availability of specific monoclonal antibodies (mabs). in the present study seven hybridoma clones producing mabs reactive with multiple subclasses of human igg were established. spleno...

The production of murine monoclonal antibody (MAb) has not yet been reported in Iran. The present work describes for the first time the generation of several murine hybridoma clones secreting MAbs directed against human leukocyte surface antigens. The secreted antibodies by hybridoma clones have been screened on different lymphoid and non-lymphoid tissues. Results indicated that of seven h...

Recent isolates of RX54-3 hybridoma cells (new cells) protect BALB/c mice against subsequent challenge with the tumorigenic myeloma parent cells used to construct this hybridoma. In contrast, hybridoma cells which have been maintained in tissue culture for long periods of time (old cells) are not protective. In the present study, we compared a number of properties of the new and old hybridoma c...

Therapeutic antibodies are implicated into the very promising and fast growing area of pharmaceutics. Human hybridoma technology, allowing generation of natural human antibodies in a native form, seems to be the most direct way that require no additional modifications for production of therapeutic antibodies. However, technical difficulties in human hybridoma creation discovered in the 80s of t...