نتایج جستجو برای: IBDV

تعداد نتایج: 315  

Journal: :Food Science and Technology 2022

Sangdu oral liquid (MFEC), a kind of traditional Chinese medicine, is used as new immune enhancer for veterinary drugs or vaccines. In current study, we aimed to evaluate the effect and mechanism MFEC on improving response B87 vaccine. Eighty SPF chickens were divided into blank control group (BC group), infectious bursal disease virus (IBDV) (IBDV vaccine (B87+IBDV group) combination (MFEC+B87...

2014
J.V.J. Silva S. Arenhart H.F. Santos S.R. Almeida-Queiroz A.N.M.R. Silva I.M. Trevisol G.R. Bertani L.H.V.G. Gil

The Infectious Bursal Disease Virus (IBDV) causes immunosuppression in young chickens. Advances in molecular virology and vaccines for IBDV have been achieved by viral reverse genetics (VRG). VRG for IBDV has undergone changes over time, however all strategies used to generate particles of IBDV involves multiple rounds of amplification and need of in vitro ligation and restriction sites. The ai...

Journal: :Avian diseases 2007
Silke Rautenschlein Christian Kraemer Enrique Montiel Johan Vanmarcke Christine Haase

Different infectious bursal disease virus (IBDV) live vaccines (intermediate, intermediate plus) were compared for their immunosuppressive abilities in specific-pathogen-free (SPF) layer-type chickens or commercial broilers. The Newcastle disease virus (NDV) vaccination model was applied to determine not only IBDV-induced immunosuppression but also bilateral effects between IBDV and NDV. None o...

Journal: :The Journal of general virology 1990
F S Kibenge D J Jackwood C C Mercado

The nucleotide sequence of genome segment A cDNA of the STC strain of infectious bursal disease virus (IBDV) was determined and compared with sequences of the homologous genome segment of the 002-73 strain of IBDV and the Jasper strain of infectious pancreatic necrosis virus (IPNV). The STC-IBDV genome segment A was determined to be 3262 base pairs (bp), which is close to the estimated total le...

2016
Ijeoma O. Nwagbo Ismaila Shittu Chika I. Nwosuh George O. Ezeifeka Frederick J. C. Odibo Linda O. Michel Daral J. Jackwood

AIM To characterize field isolates of infectious bursal disease virus (IBDV) from outbreaks in nine states in Nigeria through reverse transcription polymerase chain reaction (RT-PCR) and sequence analysis of portions of the VP2 and VP1 genes and to determine the presence or absence of reassortant viruses. MATERIALS AND METHODS A total of 377 bursa samples were collected from 201 suspected IBD...

D. Shahbazzadeh H. Goudarzi M. Azizy M.R. Seyfi Abad Shapoori R. Toroghi S.A. Pourbakhsh,

Infectious bursal disease virus (IBDV) is the causative agent of Gumboro disease, an infectious disease of global economic importance in poultry. The expression of heterologous proteins in P.pastoris is fast, simple and inexpensive. In this study, VP2 encoding gene of classical D78 IBDV was amplified using reverse transcription (RT) polymerase chain reaction (PCR) and cloned into pPICZαA vector...

2015
Nian Wang Lizhou Zhang Yuming Chen Zhen Lu Li Gao Yongqiang Wang Yulong Gao Honglei Gao Hongyu Cui Kai Li Changjun Liu Yanping Zhang Xiaole Qi Xiaomei Wang

Nonstructural protein VP4, a serine protease of infectious bursal disease virus (IBDV) that catalyzes the hydrolysis of polyprotein pVP2-VP4-VP3 to form the viral proteins VP2, VP4, and VP3, is essential to the replication of IBDV. However, the interacting partners of VP4 in host cells and the effects of the interaction on the IBDV lifecycle remain incompletely elucidated. In this study, using ...

2017
Yao Qin Zhichao Xu Yongqiang Wang Xiaoqi Li Hong Cao Shijun J. Zheng

Infectious bursal disease (IBD) is an acute, highly contagious and immunosuppressive avian disease caused by IBD virus (IBDV). Cell apoptosis triggered by IBDV contributes to the dysfunction of immune system in host. VP2 of IBDV is known to induce cell death but the underlying mechanism remains unclear. Here we demonstrate that VP2 interacts with the oral cancer overexpressed 1 (ORAOV1), a pote...

2018
Bin Wang Mengjiao Fu Yanan Liu Yongqiang Wang Xiaoqi Li Hong Cao Shijun J. Zheng

Infectious bursal disease (IBD) is an acute, highly contagious, and immunosuppressive avian disease caused by IBD virus (IBDV). MicroRNAs (miRNAs) are involved in host-pathogen interactions and innate immune response to viral infection. However, the role of miRNAs in host response to IBDV infection is not clear. We report here that gga-miR-155 acts as an anti-virus host factor inhibiting IBDV r...

Journal: :Journal of clinical microbiology 1994
L H Lee L J Ting J H Shien H K Shieh

An assay protocol based on single-tube, noninterrupted reverse transcription-PCR (RT-PCR) for the detection of infectious bursal disease virus (IBDV) is described. After the conditions for RT-PCR had been optimized, a primer set framing a region within the gene coding for IBDV VP2 protein was used to amplify a 318-bp fragment of the IBDV genome. Amplified product was detected with three strains...

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