Bioautography is a microbial detection method hyphenated with planar chromatography techniques. It is based mainly on antimicrobial or antifungal properties of analyzed substances. The review discusses three versions of bioautography, i.e. contact, immersion and direct bioautography. The more concern is given to the last one. Many applications are quoted, not only for testing various groups of ...

In this study antimicrobial effects of Salivia mirzayanii and Saliva atropatana were evaluated against six gram-positive and gram-negative bacteria by immersion bioautography, cylinder plate and tube dilution methods separately. In bioautography method, essential oils of Salvia mirzayanii and Salvia atropatana were separated on silicagel TLC plates by toluene-ethyl acetate (93-7). In cylander p...

Background & Aims: Today, the emergence of antimicrobial resistance against conventional antibiotics is increasing. Many researchers consider plants with antimicrobial properties as a good alternative. In this study, the antimicrobial activity of Ajuga chamaecistus Ging. Subsp. Scoparia (Bioss) Rech. f. on 8 bacterial strains wasinvestigated. Methods: The leaf extract was prepared by methanolic...

tlc screening and evaluation of antioxidant, antibacterial activity of onopordon macrocephalum by bioautography methodonopordon known as scotch thistle is a medicinal plant of the asteraceae family that is widely distributed in europe and asia, also it has many valuable medicinal properties as hypotensive and antitumor. thin layer chromatography (tlc) is wildly used in natural product extract a...

Medicinal plants, vegetables and fruits are the sources of huge number of bioactive lead/scaffolds with therapeutic and nutraceutical importance. Bioautography is a means of target-directed isolation of active molecules on chromatogram. Organic solvents employed in chromatographic separation process can be completely removed before biological detection because these solvents cause inactivation ...

BACKGROUND Athrixia phylicoides DC. (Asteraceae) is used medicinally in South Africa to treat a plethora of ailments, including heart problems, diabetes, diarrhoea, sores and infected wounds. It is also prepared in the form of a tea (hot decoction) taken as a refreshing, pleasant-tasting beverage with commercialization potential. METHODS Extracts of the dried ground aerial parts were prepared...