OBJECTIVE To evaluate the effectiveness of 15 minutes of immobilisation versus immediate mobilisation after intrauterine insemination. DESIGN Randomised controlled trial. Setting One academic teaching hospital and six non-academic teaching hospitals. PARTICIPANTS Women having intrauterine insemination for unexplained, cervical factor, or male subfertility. INTERVENTIONS 15 minutes of immo...

4. LIGAND 10 4.1. Direct versus indirect immobilisation 10 4.2. Covalent immobilisation 11 4.2.1. A general approach 11 4.2.2. Choice of chemistry 12 4.2.3. Prepare the protein 12 4.2.4. Pre-concentration 12 4.2.5. Amine coupling 14 4.2.6. Regeneration 15 4.2.7. Adjusting the immobilisation conditions. 16 4.3. Non-covalent immobilisation (ligand capture) 16 4.3.1. Using an existing strategy 17 ...

Common measures to establish spinal immobilisation at the scene of an accident include keeping the patient's head still, applying a rigid cervical collar and transporting the patient on a rigid spinal board to an emergency department. This article reviews the literature about spinal immobilisation practices in emergency settings, including best-practice guidance and papers on immobilisation, im...

   Management of first shoulder dislocation following reduction remains controversial. The two main options are immobilisation and arthroscopic stabilisation. The aim of this article is to highlight some of the issues that influence decision making when discussing management options with these patients, including natural history of the first time dislocation, outcomes of surgery and non-operati...

In this tutorial review, an overview of the why, what and how of enzyme immobilisation for use in biocatalysis is presented. The importance of biocatalysis in the context of green and sustainable chemicals manufacture is discussed and the necessity for immobilisation of enzymes as a key enabling technology for practical and commercial viability is emphasised. The underlying reasons for immobili...

INTRODUCTION There is a paucity of data regarding the risk of deep vein thrombosis during hip plaster cast immobilisation. The purpose of this article was to review the available evidence regarding the incidence of symptomatic venous thromboembolism (VTE) during hip plaster cast immobilisation. METHODS AND MATERIALS All papers describing hip plaster cast immobilisation published in the Englis...

OBJECTIVES To study the role of different components in normal human serum and the role of lysozyme in rapid immobilisation of Percoll purified T pallidum (Nichols). MATERIALS AND METHODS The immobilisation of Percoll purified T pallidum was studied after pre-incubations with different serum fractions (Fr) of normal human serum (Fr 1, containing IgM; Fr 2, containing IgG and a low level of ha...

BACKGROUND Horseradish Peroxidase (HRP) plays important roles in many biotechnological fields, including diagnostics, biosensors and biocatalysis. Often, it is used in immobilised form. With conventional immobilisation techniques, the enzyme adheres in random orientation: the active site may face the solid phase rather than bulk medium, impeding substrate access and leading to sub-optimal catal...

OBJECTIVES To determine whether disruption of the p53 gene leads to preservation of trabecular bone volume (BV) after limb immobilisation. MATERIALS AND METHODS Tibias of immobilised hind limbs of p53 gene knockout (p53(-/-)) and wild-type (p53(+/+)) mice were compared. Right knee joints of 8 week old mice were immobilised in full extension for 7 days. Trabecular structure and bone formation ...

The integrity of the periosteum and the interosseous membrane determine the stability of fractures of the distal ulna; this is indicated by the initial displacement. In fractures displaced by less than 50% the periosteum and interosseous membranes are largely intact; these fractures are stable and require only below-elbow immobilisation for protection and relief of pain. In fractures displaced ...