To demonstrate that two structurally similar chemicals can be extracted from a complex matrix and then separated from each other on the basis of their relative affinities for an antibody, an automated column-switching system was used, incorporating on-line, high-performance immunoaffinity chromatography (HPIAC). A high-affinity monoclonal antibody (Mab Sara-95) against the fluoroquinolone saraf...

During 2002 and 2003, a total of 107 samples of raw, pasteurized and ultrahigh treated temperature (UHT) milk commercialized in the cities of São Paulo and Marília (SP) were analyzed for the presence of aflatoxin M1 (AFM1). AFM1 was detected in 79 (73.8%) of milk samples, ranging from <0.02 to 0.26 μg/L.The samples were analyzed using an immunoaffinity column for cleanup and a thin layer chroma...

A liquid chromatographic method for the determination of ochratoxin A in grapes, dried vine fruits, and winery byproducts was developed. A mixture of either acetonitrile/water or acetonitrile/water/methanol was used as an extraction solvent mixture. After immunoaffinity column cleanup, the final extract was analyzed by high-performance liquid chromatography (HPLC) with a fluorometric detector (...

Liquid chromatography (LC) coupled with mass spectrometry (MS) is widely used for the determination of mycotoxins in cereals and cereal-based products. In addition to the regulated mycotoxins, for which official control is required, LC-MS is often used for the screening of a large range of mycotoxins and/or for the identification and characterization of novel metabolites. This review provides i...

This study investigated the natural incidence of deoxynivalenol (DON) in corn and products from corn-producing districts Punjab, Pakistan. The analysis was carried out using high performance liquid chromatography (HPLC) with UV detector immunoaffinity cleanup columns. detection limit (LOD) quantification were 25 50 µg/kg, respectively. A total 1220 samples analyzed to detect DON, 539 (44.2%) ob...

An immunoaffinity-based method was developed to determine zearalenone in corn. Corn samples were extracted in acetonitrile-water (90 + 10, v/v), applied to an immunoaffinity column, and eluted with methanol. The isolated toxin was quantitated either by reaction with aluminum chloride hexahydrate (AlCl3.6H2O) prior to measurement with a fluorometer or injection into a liquid chromatographic (LC)...

mycotoxins are small, toxic chemical products produced as secondary metabolites by a few fungal spices that readily colonise crops and contaminate them with toxins in the field or after harvest. in this study a liquid chromatographic method was developed for the simultaneous determination of aflatoxins (afs) (b 1 , b 2 , g 1 , and g2), ochratoxin a (ota) and zearalenone(zea) toxins in wheat. fo...