The transport of the nutrient secretagogue 2-ketoisocaproate (KIC) was studied in isolated rat pancreatic islets and in the HIT-T15 insulinoma cell line using an oil-filtration technique. In both islets and HIT-T15 cells, KIC uptake was a slow process, not reaching equilibrium within 10 min KIC transport was not dependent upon Na+ in the medium, was not inhibited by alpha-cyano-4-hydroxycinnama...

Kinesin-like calmodulin binding protein (KCBP) is a microtubule motor protein involved in the regulation of cell division and trichome morphogenesis. Genetic studies have shown that KCBP is likely to interact with several other proteins. To identify KCBP-interacting proteins, we used the C-terminal region of KCBP in a yeast two-hybrid screen. This screening resulted in the isolation of a novel ...

Culturing rat islets in high glucose (HG) increased 1-Cketoisocaproate (KIC) oxidation compared with culturing them in low glucose. Leucine caused insulin secretion (IS) in low glucose but not in HG rat islets, whereas KIC did so in both. Pretreatment with HG for 40 min abolished leucine stimulation of IS by mouse islets and prevented the cytosolic Ca rise without inhibiting IS and Ca increment...

Oxidation of a-ketoisocaproate (KIC, 4-methyl-2-oxopentanoate) by coupled liver mitochondria was inhibited 60-70% by 1 m~ AMP, ADP, or its analogue, adenosine-5’-0-(2-thodiphosphate); inhibition was blocked by atractyloside. AMP inhibition was mediated by ADP generated via adenylate kinase. ADP inhibition was timeand temperature-dependent and required mitochondrial integrity. Oxidation of a-ket...

In human protein turnover studies with isotopically labeled leucine (Leu) as a tracer, plasma ketoisocaproate (KIC) enrichment is extensively used as a surrogate measure of intracellular leucine enrichment. To test how accurately arterial ketoisocaproate (A-KIC) represents leucine isotopic enrichment in the hepatic (HV) and femoral veins (FV), which drain liver and muscle beds, we measured Leu ...

Plane strain fracture toughness (KIC) has been evaluated for a number of commercially-available dental composites. A modified Short-rod Fracture Toughness (SRFT) specimen design has been used, enabling small specimens to be tested conveniently. The effect on KIC of aging in water at 37 degrees C for seven days, one month, and six months has been determined for conventional, microfilled, and hyb...

BACKGROUND This study examined the efficacy of short-term alpha-ketoisocaproic acid (KIC) monotherapy supplementation immediately prior to moderate- and high-intensity single bout exercise performance. METHODS Thirteen resistance trained men (22.8 +/- 2.5 years; 81.6 +/- 12.6 kg) participated in a prospective, randomized, double blind, placebo controlled crossover experiment. Each subject com...

Kinesin-like calmodulin binding protein (KCBP) is a microtubule motor protein involved in the regulation of cell division and trichome morphogenesis. Genetic studies have shown that KCBP is likely to interact with several other proteins. To identify KCBP-interacting proteins, we used the C-terminal region of KCBP in a yeast two-hybrid screen. This screening resulted in the isolation of a novel ...

KIC 8462852 is a superficially ordinary main sequence F star for which Kepler detected an unusual series of brief dimming events. We obtain accurate relative photometry of KIC 8462852 from the Kepler full-frame images, finding that the brightness of KIC 8462852 monotonically decreased over the four years it was observed by Kepler. Over the first ∼1000 days KIC 8462852 faded approximately linear...

To improve the efficiency of wireless data communications, new physical-layer transmission methods based on known-interference cancellation (KIC) have been developed. These methods share the common idea that the interference can be cancelled when the content of it is known. Existing work on KIC mainly focuses on single-hop or two-hop networks, with physical-layer network coding (PNC) and full-d...