background methotrexate is a folic acid antagonist that has been in clinical use for five decades. its use in patients with brain tumors is primarily confined to primary central nervous system lymphoma (pcnsl), in which it is the cornerstone of chemotherapy. objectives a selective and sensitive high-performance liquid chromatography-electrospray ionization-mass spectrometry method was developed...

a convenient liquid chromatographic-single quadrupole mass spectrometric (lc-ms) method was developed and validated for dexchlorpheniramine maleate (inn name: chlorphenamine) determination in human plasma. the need for just a single liquid-liquid extraction with ethyl acetate and being highly sensitive were the advantages of this method. the linearity was also excellent over the range of 1 to 1...

background and aims: a selective and sensitive high performance liquid chromatography-electrospray ionization mass spectrometry method has been established for determination of methotrexate in human plasma. methods: methotrexate was extracted from plasma with acetonitrile. the mobile phase consisted of acetonitrile-water-formic acid 74: 25: 1(v/v). twenty µl of sample was chromatographically an...

for the first time, a multi-residue methodfor simultaneous determination of 41lc-amenable pesticides in rice, belonging to different chemical classes has been developed in iran by lc-ms/ms. the pesticides were analyzed simultaneously in a single run using positive electrospray ionization with multiple reaction monitoring (mrm) after extraction with slightly modified quechers method. the calibra...

objective: ipomoea mauritiana is used in both ayurveda and folk medicine systems. the tuberous roots are known to be diuretic, depurative, carminative, and anthelmintic. the objective of the current study was to identify phenolic antioxidants from i. mauritiana using spectrophotometric and lc-ms analysis. materials and methods: an activity-guided fractionation and purification process was used t...

twenty six rhizobium strains were isolated from root nodules of sesbania sesban (l.) merr. collected from different regions of andhra pradesh. all the 26 rhizobium strains produced indole acetic acid (iaa), but maximum amount was produced by only five strains in yeast extract mannitol  (yem) medium supplemented with l-tryptophan. the strains were found to elaborate maximum iaa when fed with 2.5...

cyanobacteria blooms are common in baltic sea and their intensity have been increased due toanthropogenic eutrophication. in this study we investigated the cyanotoxin levels in the water samplescollected from four different locations in the baltic sea at three different seasons including summer 2011.protein phosphatase 2a (pp2a) inhibition assays, enzyme-linked immunosorbent assays (elisa) and ...