A 56 days grow-out experiment with three protein levels (35, 40 and 45%) and three lipid levels (5, 10 and 15%) was conducted to evaluate the optimum protein to lipid ratio for oriental river prawn (Macrobrachium nipponense). Four hundred and five prawns, with mean (± SD) weight of 1.40 ± 0.04 g were distributed in 27 glass aquaria and were fed four times daily. Results showed tha...

The results of cytological, biochemical, and molecular biological studies clearly demonstrate the significant influence microgravity on main processes plant development, revealing mechanisms which underlie reactions responses to ensure their adaptation this factor. key role in cell function belongs cytoplasmic membrane (CM). First all, by its qualities functions, it can play an important plants...

in this study, the energy reserves of prediapause and diapausing adult bugs were examined using colorimetric biochemical techniques to determine carbohydrates, lipids, glycogen, and protein content. to this end, 45-day-old bugs were obtained from three different sources: laboratory colony, cold-stored insects, or natural habitat. the results showed that prediapause males and females had signifi...

Delipidated high density lipoprotein (apo-HDL), isolated apolipoproteins apoA-I and apoA-II, S-carboxymethylated apoA-II, apoC-III, the NH(2)- and COOH-terminal CNBr peptides of apoA-II, and the COOH-terminal CNBr peptide of apoA-I were recombined in vitro with [N-C(3)H(3)-choline]phosphatidylcholine (PC) and [N-(14)CH(3)-choline]sphingomyelin (SPM). The lipid-protein complexes were analyzed by...

Background: Protein-protein interactions do not provide any direct information re‌garding the domains within the proteins that mediate the interactions. The majority of proteins are multi domain proteins and the interaction between them is often defined by the pairs of their domains. Most of the former studies focus only on interacting do‌main pairs. However they do not consider the in...

Electron crystallography is arguably the only electron cryomicroscopy (cryoEM) technique able to deliver an atomic-resolution structure of membrane proteins embedded in the lipid bilayer. In the electron crystallographic structures of the light driven ion pump, bacteriorhodopsin, and the water channel, aquaporin-0, sufficiently high resolution was obtained and both lipid and protein were visual...

In each case the complexes were suspended in 20rn~-Tris/HCl (pH 8.0)/0.1% LDAO (v/v). The circles indicate where the recording spectrophotometer stopped and the spectra were continued point by point in another machine. (a) Complexes from strain 7750 grown at 2000k: 0, type 1 B800-850; 0, RC-B880. (b) Complexes from strain 7050 grown at 4001~: 0, type 2 B800-850; this spectrum was raised to make...

Recent experimental results revealed that lipid-mediated interactions due to hydrophobic forces may be important in determining the protein topology after insertion in the membrane, in regulating the protein activity, in protein aggregation and in signal transduction. To gain insight into the lipid-mediated interactions between two intrinsic membrane proteins, we developed a mesoscopic model of...