In the biosynthetic gene cluster of the aminocoumarin antibiotic clorobiocin, the small ORF cloY encodes a 71 aa protein which shows significant sequence similarity to mbtH from the mycobactin biosynthetic gene cluster of Mycobacterium tuberculosis. mbtH-like genes are frequently found in the biosynthetic gene clusters of peptide antibiotics and siderophores, but their function has remained eni...

Background: L-tyrosine adenylation is a key step in aminocoumarin antibiotic and vancomycin biosynthesis. Results: Several, but not all tyrosine-adenylating enzymes require MbtH-like proteins for activity, forming heterotetrameric complexes. A single L→M mutation creates an MbtH-independent enzyme. Conclusion and Significance: MbtH-like proteins are essential tools in the combinatorial biosynth...

Three peroxidase (POD) isoenzymes were purified from a soluble extract of broccoli stems. The acidic and neutral PODs were purified to homogeneity by using ion exchange and hydrophobic chromatography. The basic POD was purified by cation exchange and gel filtration chromatography. The neutral and basic PODs had molecular masses of approximately 43 kDa, and the acidic POD had a molecular mass of...

Two simple, precise and accurate spectrophotometric methods were developed for the estimation of Gefitinib in bulk drug and in pharmaceutical formulations. Method A and B is based on oxidation of followed by coupling of 3metyl-1,2-benzothiazolinone hydrazone (MBTH) with Gefitinib using Ferric chloride and Cerric ammonium nitrate(CAN) and measuring the chromogen at the λmax of 438 and 675 respec...

The evaluation of a badge-type diffusive sampler for measuring formaldehyde using 3-methyl-2-benzothiazolinone hydrazone (MBTH) was investigated. On average, the formaldehyde concentration in blanks was reduced by approximately 31% by cleaning procedures. The cleaning techniques did not significantly differ in effectiveness. The maximum sampling rate was 22.4 +/- 3.5 mL min(-1) at MBTH concentr...

We explored mined block temporarily holding (MBTH) with enforce fork (MBTH-EF) attacks to understand the effect of selfish mining on winning rate and fairness. The temporary time provides a pool additional begin next early. In this situation, may risk losing block. One method maintain probability held is ensure sends out hash values once it receives message from other pools. analyzed MBTH-EF ev...

The target of the present study is to use experimental design in screening and optimizing experimental variables to develop a spectrophotometric method for determination of Cefdinir (CFN) antibiotic. The proposed method is based on the oxidative coupling reaction of CFN with 3methylbenzothiazolinone-2hydrazone (MBTH) in presence of FeCl3 in acidic medium forming a green colored chromogen measur...

A catalytic spectrophotometric method for the determination of traces of copper(II) is proposed. 3-Methyl-2-benzothiazolinone hydrazone (MBTH) is oxidized by hydrogen peroxide to form a yellowish-brown compound. The reaction is accelerated by trace amounts of copper(II), and can be followed by measuring the increase in the absorbance at 390 nm. Since the absorbance at 40 min from the reaction s...

Three colorimetric reagents for the determination of formaldehyde, the Nash reagent (ammonia plus acetylacetone), Purpald (4-amino-3-hydrazino-5-mercapto-1,2,4-triazole), and N-methylbenzothiazolinone-2-hydrazone (MBTH), were compared for the determination of methanol when used in conjunction with alcohol oxidase. The combination of alcohol oxidase plus the commonly used Nash reagent was specif...

for the determination of serum or plasma triglycerides. The samples are extracted with 2propanol containing activated alumina. An AutoAnalyzer Sampler II and Proportioning Pump II are used in combination with a Gilford Model 300 N spectrophotometer and Model 3091-A debubbler flow cell. Following on-line saponification, glycerol is oxidatively cleaved with sodium metaperiodate. The resulting for...