Objective. To determine whether the genes whose products are annotated as «MBL-fold metallohydrolase superfamily» related to proteins of metallo-β-lactamase group. Materials and Methods. Sequences 7 were analyzed using following resources: ClustalW, IQ-TREE, iTOL. Selection oligonucleotides for real-time PCR was performed Primer-BLAST resource. The level gene expression assessed PCR. MIC MBC me...

pseudomonas aeruginosa is among the most important pathogens in the nosocomial infections. a genetic mobile element, the integron, is one of the major agents involved in dissemination of multi-drug resistance among gram negative bacteria. during a descriptive study from october 2009 to august 2010, some 130 p. aeruginosa clinical isolates were collected from different wards of three hospitals i...

background and objectives: pseudomonas aeruginosa is a frequent opportunistic pathogen in health care associated infections that is highly resistant to the majority of β-lactams. the aims of this study were to access the antimicrobial susceptibility pattern of p. aeruginosa isolated from educational hospitals of qazvin and alborz provinces, to determine the prevalence of metallo-β-lactamase (mb...

Metallo-β-lactamase-producing Pseudomonas aeruginosa (MPPA) is an important nosocomial pathogen that shows resistance to all β-lactam antibiotics except monobactams. There are various types of metallo-β-lactamases (MBLs) in carbapenem-resistant P. aeruginosa including Imipenemase (IMP), Verona integron-encoded metallo-β-lactamase (VIM), Sao Paulo metallo-β-lactamase (SPM), Germany imipenemase (...

conclusions this study showed that multiple β-lactamases can be produced in burn isolates. this suggests that use of cephalosporins and carbapenems should be restricted in burn isolates to minimize the development and spread of these multidrug resistant pathogens. results there was 99% resistance to carbenicillin, and ticarcillin, 98% to cotrimoxazole, 96% to ciprofloxacin, and aztreonam, 95% t...

conclusions our findings showed a high occurrence of metallo-β-lactamase production among p. aeruginosa isolates in burn patient infections in our region. also, there are p. aeruginosa isolates carrying the bla-vim-1 and bla-vim-2 genes in isfahan province. background metallo-β-lactamase-production among gram-negative bacteria, including pseudomonas aeruginosa, has become a challenge for treatm...

INTRODUCTION Acquired production of metallo-β-lactamases is an important mechanism of resistance in Pseudomonas aeruginosa. The objective of this study was to investigate the production of metallo-β-lactamase and the genetic diversity among ceftazidime-resistant P. aeruginosa isolates from State of Sergipe, Brazil. METHODS Metallo-β-lactamase was investigated using the disk approximation test...

β-Lactamases inactivate β-lactam antibiotics by hydrolysis of their endocyclic β-lactam bond and are a major cause of antibiotic resistance in pathogenic bacteria. The zinc dependent metallo-β-lactamase enzymes are of particular concern since they are located on highly transmissible plasmids and have a broad spectrum of activity against almost all β-lactam antibiotics. We present here essential...

We characterized 9 New Delhi metallo-β-lactamase-producing Enterobacteriaceae (5 Klebsiella pneumoniae, 2 Escherichia coli, 1 Enterobacter cloacae, 1 Salmonella enterica serovar Senftenberg) isolates identified in the United States and cultured from 8 patients in 5 states during April 2009-March 2011. Isolates were resistant to β-lactams, fluoroquinolones, and aminoglycosides, demonstrated MICs...

BACKGROUND Metallo-β-lactamase-production among Gram-negative bacteria, including Pseudomonas aeruginosa, has become a challenge for treatment of infections due to these resistant bacteria. OBJECTIVES The aim of the current study was to evaluate the metallo-β-lactamase-production and carriage of bla-VIM genes among carbapenem-resistant P. aeruginosa isolated from burn wound infections. PATI...