background: dna extraction is one the most important steps for molecular analysis of food-borne pathogens. in this research, three methods of dna extractions from beef harboring sarcocystis spp. were compared for the quality, quantity, safety, as well as cost-effectiveness. methods: about 100 mg intersostal and diaphragm were collected from 10 slaughtered cattle. after ensuring their contaminat...

background: sarcocystosis is one of the most widespread parasitic diseases in cattle caused by three main species of sarcocystis genus. the major aim of this study was to determine the prevalence of sarcocystis spp. in meat produced in yazd, central iran with special reference to species identification, using polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp).  method...

introduction: the main agents of sarcocystosis in cattle as an intermediate host include s. cruzi, s. hominis and s. hirsuta. a sensitive and specific tool such as molecular-based techniques would be necessary to identify the species. case report: after collection of beef sample from yazd slaughterhouse, dna extraction was done with salting out method. the 18srrna gene as a specific target gene...

conclusions despite rapidity, automation, accuracy, cost-effectiveness, high sensitivity, and high specificity of molecular techniques, each type of molecular technology has its own advantages and disadvantages. results various types of molecular biology tools can be used to detect and quantify viral genomic elements and analyze the sequences. these molecular assays are proper technologies for ...

personalized medicine is significantly changing the fundamentals of healthcare. genetic diagnostic tests are used in various parts of medicine – from initial screening to the diagnosis and finally treatment. investigations in fields of drug development indicate that oncology may lead other therapeutic areas in the number of targeted therapies on the marketplace as well as in the pipeline, expec...

various molecular techniques are available to make differences and to study the genetic divergences among species and populations, of which the most efficient ones are pcr-based markers. one of these methods, molecular fingerprinting, enables the distinction of closely related populations. issr-primers are universal primers that can be used for such studies. the utility of these primers was tes...

background: group b streptococcus (gbs) is the major cause of serious life threatening infections in neonates, pregnant women, and other adults with underlying diseases. capsular polysaccharide typing is a significant way for epidemiological studies of gbs, the pathogenesis, and other studies associated with gbs infections including surveillance programs and vaccine development in future. molec...

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