introduction non-invasive fluorescent reflectance imaging (fri) is used for accessing physiological and molecular processes in biological media. the aim of this article is to separate the overlapping emission spectra of quantum dots within tissue-equivalent phantom using svd, jacobi svd, and nmf methods in the fri mode. materials and methods in this article, a tissue-like phantom and an optical...

A variety of multi-spectral imaging methods are discussed for acquiring spectral information from a scene. We first review conventional multispectral imaging approach. The conventional imaging systems are mostly constructed by multi-band imaging devices with different filtration mechanism at the sensor side under passive illumination. We show some imaging devices, estimation algorithms, and app...

Multispectral imaging is highly demanded for precise color reproduction and for various computer vision applications. Multispectral imaging with a multispectral color filter array (MCFA), which can be considered as a multispectral extension of commonly used consumer RGB cameras, could be a simple, low-cost, and practical system. A challenge of the multispectral imaging with the MCFA is multispe...

LED illumination based multispectral imaging is getting much attention in recent years due to its fast computer controlled switching ability, availability of many different LEDs, robustness, and cost effectiveness. In this paper, we propose a system which uses an RGB camera along with two or three combinations of three different types of LEDs in order to acquire multispectral images of six or n...

Spectral imaging including machine vision and multispectral imaging can provide a rapid, nondestructive means to assess agricultural commodities for their quality and safety for human consumption. These nondestructive techniques generally rely on reflectance measurements; the most commonly used spectral regions range through the visible (Vis) to the near infrared (NIR). Another optical sensing ...

Current multispectral imaging systems use narrowband filters to capture the spectral content of a scene, which necessitates different filters to be designed for each application. In this paper, we demonstrate the concept of Fourier multispectral imaging which uses filters with sinusoidally varying transmittance. We designed and built these filters employing a single-cavity resonance, and made s...

Multispectral imaging is a powerful tool that extends the capabilities of the human eye. However, multispectral imaging systems generally are expensive and bulky, and multiple exposures are needed. Here, we report the demonstration of a compact multispectral imaging system that uses vertical silicon nanowires to realize a filter array. Multiple filter functions covering visible to near-infrared...

The color accuracy of conventional RGB cameras is not sufficient for many color-critical applications. One of these applications, namely the measurement of color defects in yarns, is why Prof. Til Aach and the Institute of Image Processing and Computer Vision (RWTH Aachen University, Germany) started off with multispectral imaging. The first acquisition device was a camera using a monochrome se...

Counting of different classes of white blood cells in bone marrow smears can provide pathologists with valuable information regarding various hematological disorders. For automatic imaging analysis techniques, precise segmentation of White Blood Cells is quite challenging due to the complex contents in bone marrow smears. Far more different from traditional color imaging analysis method, we int...