determination of streptokinase activity is usually accomplished through two assay methods: a) clot lysis, b) chromogenic substrate assay. in this study the biological activity of two streptokinase products, namely streptase®, which is a native product and heberkinasa®, which is a recombinant product, was determined against the third international reference standard using the two forementioned a...

When streptokinase is incubated with human or rabbit plasminogen, one event which occurs is a specific fragmentation of streptokinase. At least five major identifiable streptokinase fragments appear with time, and they possess molecular weights of approximately 40,000 (SK I), 36,000 (SK 2), 31,000 (SK 3), 26,000 (SK 4), and 10,000 (SK 5) under denaturing conditions, as observed on calibrated so...

Methods are described for measuring plasminogen levels in human plasma and for studying plasmin generation in plasma with plasminogen activators. In plasma samples from two individuals with histories of deep vein thrombosis, the functional plasminogen values were determined to be below the normal range of 16.7 to 23.8 mg/lOO ml (average of 21.0 mg/lOO ml), and the plasmin generation rates were ...

Methods are described for measuring plasminogen levels in human plasma and for studying plasmin generation in plasma with plasminogen activators. In plasma samples from two individuals with histories of deep vein thrombosis, the functional plasminogen values were determined to be below the normal range of 16.7 to 23.8 mg/lOO ml (average of 21.0 mg/lOO ml), and the plasmin generation rates were ...

AIMS The dosage of fibrinolytic agents such as streptokinase must be controlled carefully to maximize therapeutic activity while avoiding adverse effects. Therefore, the integrity and activity of streptokinase products is likely to be clinically relevant. This study was conducted to compare the in vitro characteristics of different streptokinase preparations. METHODS AND RESULTS Sixteen strep...

Determination of streptokinase activity is usually accomplished through two assay methods: a) Clot lysis, b) Chromogenic substrate assay. In this study the biological activity of two streptokinase products, namely Streptase®, which is a native product and Heberkinasa®, which is a recombinant product, was determined against the third international reference standard using the two forementioned a...

Determination of streptokinase activity is usually accomplished through two assay methods: a) Clot lysis, b) Chromogenic substrate assay. In this study the biological activity of two streptokinase products, namely Streptase®, which is a native product and Heberkinasa®, which is a recombinant product, was determined against the third international reference standard using the two forementioned a...

BACKGROUND Worldwide, streptokinase remains the most used thrombolytic agent for the treatment of myocardial infarction. Recombinant streptokinase, from E. coli, is increasingly used in developing countries as a biosimilar of native streptokinase; however, potency assignments relative to the WHO International Standard (IS) are highly variable with potentially dangerous consequences. A proportio...

Streptokinase is shown to react with either human plasmin or human plasminogen to form an equimolar product which migrates in starch gel electrophoresis at pH 6.0 or 8.0 with a migration rate intermediate between the rates of migration of the two proteins. When streptokinase was present in molar excess in the reaction mixtures, the uncombined streptokinase displayed a migration rate at pH 6.0 (...

DETERMINATION OF STREPTOKINASE ACTIVITY IS USUALLY ACCOMPLISHED THROUGH TWO ASSAY METHODS: a) Clot lysis, b) Chromogenic substrate assay. In this study the biological activity of two streptokinase products, namely Streptase®, which is a native product and Heberkinasa®, which is a recombinant product, was determined against the third international reference standard using the two forementioned a...