Extracellular and intracellular copper and zinc regulate synaptic activity and plasticity, which may impact brain functionality and human behavior. We have found that a metal coordinating molecule, Neocuproine, transiently increases free intracellular copper and zinc levels (i.e., min) in hippocampal neurons as monitored by Phen Green and FluoZin-3 fluorescence, respectively. The changes in fre...

A precise automated procedure developed for determination of serum lactate dehydrogenase, using the Robot Chemist, measures absorbance of a cuprous-neocuproine complex formed by coupled reduction of the cupric-neocuproine reagent with enzymatically generated reduced nicotinamide adenine dinucleotide. Activity values obtained by this method, by the identical automated colorimetric method on the ...

Simple, sensitive and selective spectrophotometric methods were developed for the determination of five antiulcer drugs namely, omeprazole (OMZ), lansoprazole (LNZ), pantoprazole (PNZ), rabeprazole (RBZ) and esomeprazole (EMZ) using neocuproine or bathocuproine as reagents. The reaction is based on the reduction of copper(II) to copper(I) by OMZ, LNZ, PNZ, RBZ and EMZ which subsequently reacts ...

(a) HA; (b) hydrolytic product of testicular, lysosomal or venom HYASE; (c) hydrolytic product of leech HYASE; (d) hydrolytic product of bacterial HYASE. sensitivity and the production of interfering precipitates, the Morgan-Elson assay cannot be used for kinetic investigation of the enzyme. Furthermore this assay is prone to interference from compounds such as disodium cromoglycate, (+ )-catec...

Glucose was measured by the ferricyanide, the Beckman glucose oxidase, and the hexokinase procedures in 228 plasma samples taken during standard oral glucose-tolerance tests in 17 normal subjects and in 21 chemical diabetics. The neocuproine method was also used to measure glucose concentration in 156 samples (78 before and 78 after dialysis) collected from six diabetic and uremic patients who ...

This article presents a reliable, effective and easy procedure for measuring glutathione peroxidase (Gpx) activity. Enzyme samples were incubated with phosphate buffer, which included the appropriate concentrations of (GSH) peroxide as substrates, to determine Gpx In CUPRAC method, reagent Cu(Nc)22+ was added stop enzymatic reaction after sufficient period incubation. The unreacted substrates a...

Effects of o-phenanthroline, 2,2'-dipyridyl and neocuproine, which form stable complexes preferentially with Fe(II), Fe(II) and specifically with Cu(I), respectively, on the inhibitory activity of bleomycin against DNA synthesis of rat ascites hepatoma AH66 cells were examined. The inhibitory activity of metal-free bleomycin was suppressed in the presence of o-phenanthroline or 2,2'-dipyridyl, ...

Palladium/neocuproine catalyzed oxidation of glucosides shows an excellent selectivity for the C3-OH, but in mannosides and galactosides, unselective oxidation was initially observed. For further application in more-complex (oligo)saccharides, a better understanding of the reaction, in terms of selectivity and reactivity, is required. Therefore, a panel of different glycosides was synthesized, ...

A novel simple kinetic spectrophotometric method for the determination of N-acetyl-L-cysteine (NAC) has been developed and validated. The proposed method is based on the reduction of Cuneocuproine reagent to Cu-neocuproine with the analyte, in a Britton-Robinson buffer solution (pH = 3.0). The non-steady state absorbance of the formed Cu-neocuproine complex is measured at 458 nm. The initial ra...