We have cloned and sequenced a DNA fragment down stream from the nifHIDK operon (1) from C. pasteurianum W5. The deduced amino acids of an ORF in this fragment of DNA shares sequence similarity of 33% with that of nifE gene from Azotobacter vinelandii (2). Therefore we designate this gene as nifE of C. pasteurianum. The identical amino acids are underlined in the figure. There was no other codi...

Detailed microstructural investigation of ion beam deposited giant magnetoresistance ~GMR! spin valves has been carried out using various techniques of transmission electron microscopy ~TEM! and x-ray diffraction. Two fcc phases, i.e., FeMn and NiFe/Co/Cu/Co/NiFe have been identified in ion-beam deposited Ta ~50 Å!/FeMn ~80 Å!/NiFe~30 Å!/Co~15 Å!/Cu~33 Å!/Co~15 Å!/NiFe~60 Å!/Ta~25 Å!/Si~001! sp...

BACKGROUND Hydrogenases catalyze reversible reaction between hydrogen (H2) and proton. Inactivation of hydrogenase by exposure to oxygen is a critical limitation in biohydrogen production since strict anaerobic conditions are required. While [FeFe]-hydrogenases are irreversibly inactivated by oxygen, it was known that [NiFe]-hydrogenases are generally more tolerant to oxygen. The physiological ...

Most methanogenic archaea reduce CO(2) with H(2) to CH(4). For the activation of H(2), they use different [NiFe]-hydrogenases, namely energy-converting [NiFe]-hydrogenases, heterodisulfide reductase-associated [NiFe]-hydrogenase or methanophenazine-reducing [NiFe]-hydrogenase, and F(420)-reducing [NiFe]-hydrogenase. The energy-converting [NiFe]-hydrogenases are phylogenetically related to compl...

OBJECTIVE Preeclampsia (PE) is a complication affecting pregnant women worldwide, which usually manifests as severe maternal hypertension. Resveratrol (RESV), a naturally existing polyphenol, is known to exhibit beneficial effects in cardiovascular disease including hypertension. We evaluated the outcome of treatment combining oral nifedipine (NIFE) and RESV against PE. DESIGN AND METHODS Usi...

We describe conventional and high-resolution transmission electron microscopy ~HRTEM! characterization of the microstructure of sputtered NiFe/Cu giant magnetoresistance spin valves ~Cu/FeMn/NiFe/Cu/NiFe! sandwiched between thick Nb contact layers. Six spin valves, sputtered at different temperatures, three with thin ~3 nm! and three with thick ~24 and 30 nm! NiFe layers, were studied. All of t...

A dinuclear synthetic model of the [NiFeSe] hydrogenase active site and a structural, spectroscopic and electrochemical analysis of this complex is reported. [NiFe('S2Se2')(CO)3] (H2'S2Se2' = 1,2-bis(2-thiabutyl-3,3-dimethyl-4-selenol)benzene) has been synthesized by reacting the nickel selenolate complex [Ni('S2Se2')] with [Fe(CO)3bda] (bda = benzylideneacetone). X-ray crystal structure analys...

E-field control of interfacial exchange coupling and deterministic switching of magnetization have been demonstrated in two sets of ferromagnetic(FM)/antiferromagnetic(AFM)/ferroelectric(FE) multiferroic heterostructures, including NiFe/NiCoO/glass/PZN-PT (011) and NiFe/FeMn/glass/PZN-PT (011). We designed this experiment to achieve exchange bias tuning along the magnetic easy axis, which is cr...

The magnetic properties of a thin film consisting of an exchange-coupled [Co/Ni]2/NiFe multilayer has been studied as a function of the NiFe thickness by using Monte Carlo modeling and compared with experimental results of [Co/Ni]4/Co/NiFe multilayers. Both modeling and experiment showed that the NiFe thickness controls the effective anisotropy. The direction of the easy axis is determined by a...

Highly active, durable, and cost-effective electrocatalysts for water oxidation to evolve oxygen gas hold a key to a range of renewable energy solutions, including water-splitting and rechargeable metal-air batteries. Here, we report the synthesis of ultrathin nickel-iron layered double hydroxide (NiFe-LDH) nanoplates on mildly oxidized multiwalled carbon nanotubes (CNTs). Incorporation of Fe i...