نتایج جستجو برای: P5CS

تعداد نتایج: 130  

Journal: :Indian journal of biochemistry & biophysics 2005
N L Choudhary R K Sairam A Tyagi

The 45-days-old seedlings of drought resistant (N-22, CR143-2-2) and susceptible rice (Oryza sativa L.) genotypes (Panidhan, Pusa-169) were subjected to osmotic stress in PEG-6000 solution of -10 and -16 bar and the relative water content (RWC), proline content, and pyrroline-5-carboxylate synthetase (P5CS) activity and its P5CS expression were studied. A gradual decrease in RWC was observed in...

Journal: :American journal of human genetics 2015
Björn Fischer-Zirnsak Nathalie Escande-Beillard Jaya Ganesh Yu Xuan Tan Mohammed Al Bughaili Angela E Lin Inderneel Sahai Paulina Bahena Sara L Reichert Abigail Loh Graham D Wright Jaron Liu Elisa Rahikkala Eniko K Pivnick Asim F Choudhri Ulrike Krüger Tomasz Zemojtel Conny van Ravenswaaij-Arts Roya Mostafavi Irene Stolte-Dijkstra Sofie Symoens Leila Pajunen Lihadh Al-Gazali David Meierhofer Peter N Robinson Stefan Mundlos Camilo E Villarroel Peter Byers Amira Masri Stephen P Robertson Ulrike Schwarze Bert Callewaert Bruno Reversade Uwe Kornak

Progeroid disorders overlapping with De Barsy syndrome (DBS) are collectively denoted as autosomal-recessive cutis laxa type 3 (ARCL3). They are caused by biallelic mutations in PYCR1 or ALDH18A1, encoding pyrroline-5-carboxylate reductase 1 and pyrroline-5-carboxylate synthase (P5CS), respectively, which both operate in the mitochondrial proline cycle. We report here on eight unrelated individ...

Journal: :The Plant journal : for cell and molecular biology 2008
Gyöngyi Székely Edit Abrahám Agnes Cséplo Gábor Rigó Laura Zsigmond Jolán Csiszár Ferhan Ayaydin Nicolai Strizhov Jan Jásik Elmon Schmelzer Csaba Koncz László Szabados

Delta-1-pyrroline-5-carboxylate synthetase enzymes, which catalyse the rate-limiting step of proline biosynthesis, are encoded by two closely related P5CS genes in Arabidopsis. Transcription of the P5CS genes is differentially regulated by drought, salinity and abscisic acid, suggesting that these genes play specific roles in the control of proline biosynthesis. Here we describe the genetic cha...

Journal: :Plant physiology 1998
T Fujita A Maggio M Garcia-Rios R A Bressan L N Csonka

We isolated two tomato (Lycopersicon esculentum) cDNA clones, tomPRO1 and tomPRO2, specifying Delta1-pyrroline-5-carboxylate synthetase (P5CS), the first enzyme of proline (Pro) biosynthesis. tomPRO1 is unusual because it resembles prokaryotic polycistronic operons (M.G. García-Ríos, T. Fujita, P.C. LaRosa, R.D. Locy, J.M. Clithero, R.A. Bressan, L.N. Csonka [1997] Proc Natl Acad Sci USA 94: 82...

Journal: :Plant physiology and biochemistry : PPB 2008
Claudia O Silva-Ortega Ana E Ochoa-Alfaro Juan A Reyes-Agüero Gerardo A Aguado-Santacruz Juan F Jiménez-Bremont

Proline (Pro) is one of the most accumulated osmolytes in salinity and water deficit conditions in plants. In the present study, we measured the Pro content, the activity and the expression level of delta 1-pyrroline-5-carboxylate synthetase (P5CS: gamma-glutamyl kinase, EC 2.7.2.11 and glutamate-5-semialdehyde dehydrogenase, EC 1.2.1.41), a key regulatory enzyme involved in the biosynthesis of...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 1992
C A Hu A J Delauney D P Verma

Many plants synthesize and accumulate proline in response to osmotic stress. Despite the importance of this pathway, however, the exact metabolic route and enzymes involved in the synthesis of proline in plants have not been unequivocally identified. We report here the isolation of a mothbean (Vigna aconitifolia) cDNA clone encoding a bifunctional enzyme, delta 1-pyrroline-5-carboxylate synthet...

Under osmotic stresses, proline accumulation is an important response of plants to these conditions. Proline is a compatible osmolyte which affects many cellular and molecular aspects of plant in both normal and stressful situations. Proline is shown to be involved in plant development in normal condition and in conferring resistance to plant under biotic and abiotic stresses. Therefore, many s...

Journal: :The Plant cell 2002
Surasak Siripornadulsil Samuel Traina Desh Pal S Verma Richard T Sayre

Pro has been shown to play an important role in ameliorating environmental stress in plants and microorganisms, including heavy metal stress. Here, we describe the effects of the expression of a mothbean delta(1)-pyrroline-5-carboxylate synthetase (P5CS) gene in the green microalga Chlamydomonas reinhardtii. We show that transgenic algae expressing the mothbean P5CS gene have 80% higher free-Pr...

Journal: :Plant & cell physiology 2008
Li Ping Zhang Surya Kant Mehta Zhao Pu Liu Zhi Min Yang

Excess copper affects the growth and metabolism of plants and green algae. However, the physiological processes under Cu stress are largely unknown. In this study, we investigated Cu-induced nitric oxide (NO) generation and its relationship to proline synthesis in Chlamydomonas reinhardtii. The test alga accumulated a large amount of proline after exposure to relatively low Cu concentrations (2...

احد یامچی, , امیر موسوی, , سیروس قبادی, , فردوس رستگار جزی, , علی اصغر کارخانه‌ای, ,

Proline as a key osmoregulating solute in plants plays an overriding role in osmotic pressure adjustment of the cell under water stress conditions. In plant, a bifunctional enzyme delta-1-pyrroline-5-carboxylate synthetase (p5cs) promotes and directs proline synthesis during drought stress conditions. The activity of this enzyme is strongly induced to increase proline concentration within the c...

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