نتایج جستجو برای: Percoll

تعداد نتایج: 990  

2016
Michiko NAKAI Shun-ichi SUZUKI Junya ITO Dai-ichiro FUCHIMOTO Shoichiro SEMBON Junko NOGUCHI Akira ONISHI Naomi KASHIWAZAKI Kazuhiro KIKUCHI

In pigs, the damaged sperm membrane leads to leakage of phospholipase C-ζ (PLCζ), which has been identified as a sperm factor, and a reduction of oocyte-activating ability. In this study, we investigated whether sperm selected by Percoll gradient centrifugation (Percoll) have sufficient PLCζ, and whether the efficiency of fertilization and blastocyst formation after intracytoplasmic sperm injec...

Journal: :Reproduction, nutrition, development 1993
S Hamamah M Lanson C Barthelemy M A Garrigue J Lansac J P Muh D Royere

In order to evaluate the role of human follicular fluid (HFF) on the fertilizing capacity of spermatozoa, we studied the effect of HFF on the lipid composition and on the movement characteristics of human spermatozoa. Spermatozoa (spz) from normospermic patients were prepared with a discontinuous Percoll gradient and incubated in Ménézo B2 medium with or without a supplement of 20% HFF (HFF-Per...

Journal: :The Biochemical journal 1982
J S Wakefield J S Gale M V Berridge T W Jordan H C Ford

Peritoneal macrophages from mice, isolated rat liver Kupffer cells and rat testis Leydig cells ingested large numbers of Percoll particles, a gradient medium widely used for separation of cells and subcellular organelles by density-gradient centrifugation. A decrease in the percentage of macrophages adhering to plastic also occurred after exposure of the cells to Percoll, even at 4 degrees C, a...

Journal: :avicenna journal of medical biotechnology 0

background: the well documented source for adult multipotent stem cells is spermatogonial stem cells (sscs). they are the foundation of spermatogenesis in the testis throughout adult life by balancing self-renewal and differentiation. the aim of this study was to assess the effect of percoll density gradient and differential plating on enrichment of undifferentiated type a spermatogonia in diss...

Journal: :Journal of immunological methods 1996
M D Rozell R A Erger T B Casale

Disparate reports exist on the eosinophil chemotactic capacity of interleukin-8 (IL-8). We hypothesized that the difference is due to the methods used to purify eosinophils. We therefore compared the eosinophilotactic capacity of IL-8 on human cells isolated by Percoll (positive selection) vs. magnetic cell separation system (MACS) (negative selection). Discontinuous Percoll gradients were prec...

Journal: :Animal reproduction science 2011
C Matás L Vieira F A García-Vázquez K Avilés-López R López-Úbeda J A Carvajal J Gadea

In this study, different combinations of 2-step, discontinuous gradient centrifugation were used, consisting of three different combinations of isotonic Percoll (45/60, 60/75 and 45/90%) that allowed us to select different sperm subpopulations from fertile and normozoospermic boars. Our objective in this study is to evaluate the effects of centrifugation through three different discontinuous Pe...

2012
A. M. Batista S. V. Silva A. T. Soares

Frozen-thawed semen from six Boer bucks was pooled and used to compare the recently available commercial product CapriPure with Percoll for sperm quality. Sperm quality parameters such as sperm motility, concentration, membrane integrity (PI/CFDA), acrosome integrity (FITC-PNA) and mitochondrial activity (JC-1) were evaluated before and after sperm processing using CapriPure and Percoll density...

2014
Nutthee AM-IN Vibuntita CHANKITISAKUL Mongkol TECHAKUMPHU

The aim of this study was to evaluate sperm quality following separation and selection using a silane-coated silica colloid method (PureSperm ® ) or Percoll ® . Frozen/thawed sperm from buffalo bulls (n=5) were assigned to two groups. The first group was separated using a Percoll ® discontinuous gradient (45 and 90%) and the second group by a single layer of PureSperm ® . Sperm motility, concen...

2014
Banafsheh Heidari Minoo Gifani Abolfazl Shirazi Amir-Hassan Zarnani Behzad Baradaran Mohammad Mehdi Naderi Bahareh Behzadi Sara Borjian-Boroujeni Ali Sarvari Niknam Lakpour Mohammad Mehdi Akhondi

BACKGROUND The well documented source for adult multipotent stem cells is Spermatogonial Stem Cells (SSCs). They are the foundation of spermatogenesis in the testis throughout adult life by balancing self-renewal and differentiation. The aim of this study was to assess the effect of percoll density gradient and differential plating on enrichment of undifferentiated type A spermatogonia in disso...

Journal: :Molecular human reproduction 1998
A A Zalata A B Christophe C E Depuydt F Schoonjans F H Comhaire

The lipid composition of the sperm membrane has a significant effect upon the functional characteristics of spermatozoa. In the present study we investigated the fatty acid (FA) composition of subpopulations of spermatozoa separated on a discontinuous Percoll gradient (47:90%) and the FA composition of phospholipids (PL) of sperm heads and tails in both normal and abnormal semen samples. In nor...

نمودار تعداد نتایج جستجو در هر سال

با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید