To study the autophagy and mechanism of phosphatidylinositol-3-kinase/protein kinase B/mammalian target rapamycin signaling pathway in directional differentiation mesenchymal stem cells to provide basic research results for its application medical field is main objective study. The lung bone marrow mice were used as experimental materials. materials divided into five groups according settings n...

Nobiletin is considered a phyto-flavonoid extracted from citrus peel and shown to several pharmacological roles such as anti-inflammatory, anti-tumor neuroprotective properties. In this study, we investigate the role of nobiletin combined docetaxel enhances apoptosis in colorectal cancer cell lines. Our results confirmed enhanced docetaxel-induced reactive oxygen species generation human carcin...

Low-density (LD) lipoproteins inhibit phytohaemagglutinin-enhanced turnover of phosphatidylinositol in human peripheral lymphocytes. Turnover was assessed by 32p incorporation into phospholipids and by loss of 32p from [32P]phosphatidylinositol. Inhibition of lipid turnover by LD lipoproteins is not the result of a change in the amount of phytohaemagglutinin required for maximum cellular respon...

The content and composition of classes molecular species phospholipid extracted from Dalbergia tonkinensis Prain (Cây Sưa) seed oil in Viet Nam have been investigated using HPLC-HRMS LCMS-IT-TOF. subclasses their D. for the first time. results showed that contains all basic lipid: polar lipid, sterol, triacylglycerol, free fatty acid, diacylglycerol, hydrocarbon, wax. In which, triacylglycerol ...

The effects of Ca2+ and acetylcholine on the degradation and synthesis of phosphatidylinositol in rabbit vas deferens was studied in vitro by a pulse--chase technique and by measuring the content of the phospholipid in the tissue. Ca2+-dependent degradation of phosphatidylinositol was found in slices and homogenates prelabelled with myo-[2-3H]inositol. The phosphatidylinositol content of the sl...

The regulation of autophagy-dependent lysosome homeostasis in vivo is unclear. We showed that the inositol polyphosphate 5-phosphatase INPP5K regulates autophagic reformation (ALR), a recycling pathway, muscle. hydrolyzes phosphatidylinositol-4,5-bisphosphate [PI(4,5)P2] to phosphatidylinositol 4-phosphate [PI(4)P], and mutations cause muscular dystrophy by unknown mechanisms. report loss muscl...

PtdIns is synthesized at the endoplasmic reticulum and its intracellular distribution to other organelles can be facilitated by lipid transfer proteins [PITPs (phosphatidylinositol transfer proteins)]. In this review, I summarize the current understanding of how PITPs are regulated by phosphorylation, how can they dock to membranes to exchange their lipid cargo and how cells use PITPs in signal...

We have cloned the gene, vps34+, from the fission yeast Schizosaccharomyces pombe which encodes an 801 amino acid protein with phosphatidylinositol 3-kinase activity. The S. pombe Vps34 protein shares 43% amino acid sequence identity with the Saccharomyces cerevisiae Vps34 protein and 28% identity with the p110 catalytic subunit of the mammalian phosphatidylinositol 3-kinase. When the vps34+ ge...

Abstract Phospholipids are used for food products, drug formulas, stabilizers, lubricants, cosmetics, pharmaceuticals, and emulsifiers. Synthetic phospholipids tend to be utilized medicinal purposes, but nowadays consumer concern has shifted from natural ingredients. have functional properties towards health, often requires containing certain fatty acids such as omega 3. The purpose of this res...

The phosphatidylinositol phosphate kinase (PIPK) family of enzymes is primarily responsible for converting singly phosphorylated phosphatidylinositol derivatives to phosphatidylinositol bisphosphates. As such, these kinases are central to many signaling and membrane trafficking processes in the eukaryotic cell. The three types of phosphatidylinositol phosphate kinases are homologous in sequence...