A system suitable for real-time measurement of oxygen partial pressure (p02) in respiratory gas (oxigraphy) that is based on oxygen's ability to quench the phosphorescence of a lumiphore is described A description of currently used p02 technologies is provided followed by a description of the luminescence quenching p02 technology. A comparison of the technologies is made. The description of lum...

This study describes the use of two-photon excitation phosphorescence lifetime measurements for quantitative oxygen determination in vivo. Doubling the excitation wavelength of Pd-porphyrin from visible light to the infrared allows for deeper tissue penetration and a more precise and confined selection of the excitation volume due to the nonlinear two-photon effect. By using a focused laser bea...

Multiphoton microscopy (MPM) is widely used for optical sectioning deep in scattering tissue, in vivo [1-2]. Phosphorescence lifetime imaging microscopy (PLIM) [3] is a powerful technique for obtaining biologically relevant chemical information through Förster resonance energy transfer and phosphorescence quenching [4-5]. Point-measurement PLIM [6] of phosphorescence quenching probes has recent...

Phosphorescence in ethanol at 77 K. of the cyclophanes 1 1 4 has been investigated. It is shown that the phosphorescence characteristics are strongly determined by transannular interaction between the benzene rings. This interaction does not occur in cyclophanes 9 and 14. Phosphorescence quenching by silver Perchlorate of paracyclophanes and benzene derivatives 15-20 is due to the formation of ...

Mik, Egbert G., Ton G. van Leeuwen, Nicolaas J. Raat, and Can Ince. Quantitative determination of localized tissue oxygen concentration in vivo by two-photon excitation phosphorescence lifetime measurements. J Appl Physiol 97: 1962–1969, 2004. First published July 9, 2004; doi:10.1152/japplphysiol.01399.2003.—This study describes the use of two-photon excitation phosphorescence lifetime measure...

L-cysteine–capped Mn-doped ZnS quantum dots (QDs) were used for the determination of glutamic acid in foodstuffs. This method is based on measurement of the quenching of the phosphorescence intensity of the QDs after interacting with glutamic acid. A linear response was observed from 50 to 500 ng mL−1 glutamic acid with a limit of detection of 6.79 ng mL−1 . Room temperature phosphorescence (RT...

Partial mercuration of DNA, such that roughly one mercuric ion is bound for every two thymine residues, results in an enhancement of the phosphorescence by a factor of ten and a small enhancement of the photosteady thymine dimer yield. Complete mercuration of DNA [one Hg(II) added per momoner unit] results in quenching of the phosphorescence intensity and an inhibition of thymine dimer producti...

Stable room-temperature phosphorescence of guest aromatic molecules was achieved by the effective suppression of oxygen quenching. The organic capsule (first wall) suppressed static oxygen quenching by enclosing a guest molecule, and dynamic quenching via the capsule opening-closing process was well suppressed and manipulated by the intercalation of this capsule into the restrictive space betwe...

Thiocarbonyl compounds possess unusual photophysical properties: they fluoresce from S(2), phosphoresce from T(1) only at extremely low concentrations in solution at room temperature, have unit quantum yield of intersystem crossing from S(1) to T(1), undergo self-quenching at diffusion-controlled rates, and are quenched by ground-state oxygen leading to self-destruction. In this article, we are...

Room temperature phosphorescence emission of erythrosin B in sol±gel silica is studied over the oxygen pressure range from 10ÿ6 to 1000 mbar. Quenching by oxygen is signi®cant over the entire range. The Stern±Volmer (SV) intensity data can be very well ®tted using the two-site model. The temperature dependence of the phosphorescence and the delayed ̄uorescence are examined from ÿ173 to 2008C at...