The sequential deposition of chitosan and pectin on a supported phospolipid bilayer was examined using a quartz crystal microbalance. Multilayer formation was observed. The coating of phospholipid-stabilized emulsion droplets through centrifugation in a stepped sucrose gradient, containing successive layers of pectin and chitosan, was then investigated. Microelectrophoresis of the emulsion drop...

In this study, we isolated and characterized bacterial strains from ancient (Neogene) permafrost sediment that was permanently frozen for 3.5 million years. The sampling site was located at Mammoth Mountain in the Aldan river valley in Central Yakutia in Eastern Siberia. Analysis of phospolipid fatty acids (PLFA) demonstrated the dominance of bacteria over fungi; the analysis of fatty acids spe...

Almost 2 decades have passed since the discovery that pregnancy is associated with a basal inflammatory state involving neutrophil activation, and that this is more overt in cases with preeclampsia, than in instances with sepsis. This pivotal observation paved the way for our report, made almost a decade ago, describing the first involvement of neutrophil extracellular traps (NETs) in a non-inf...

Factor VII clotting activity increases about five-fold when blood is clotted in glass. Prior studies suggested that this results from activation induced by activated factor IX (IXa). However, in purified systems containing phospholipid and calcium, activated factor X (Xa) is known to activate factor VII rapidly. Therefore, we studied activation of factor VII by IXa and X, in systems using purif...

Indolicidin is a cationic, 13-residue antimicrobial peptide (ILPWKWPWWPWRR-NH(2)) which is unusually rich in tryptophan and proline. Its antimicrobial action involves the bacterial cytoplasmic membrane. Fluorescence and circular dichroism spectra demonstrated the structural similarity of indolicidin in complexes with large unilamellar phospolipid vesicles and with detergent micelles. The struct...

Phospholipid metabolism in the lung was studied because the lung was the first organ at which the ingested lipids arrived. It was revealed in this study that the lung contained phosphatidyl cho-line, ethanolamine, phosphoinositol, sphingomyelin and lysolecithin, that the amount of phosphatidyl serine were very small in the lung, and that a phosphatidic acid was discovered in the lung after the ...

Introduction: It is well known that tumors have an abnormal phospolipid metabolism[1]. This is typically reflected in the higher phosphomonoester and total choline compound levels observed by in vivo P and H MRS respectively, which may be used as biomarkers for diagnosis and treatment evaluation [1,2,3,4]. For practical and sensitivity reasons H MRS has been used most extensively in the clinic ...

In the present paper, we have studied immunglobulin (IgG) of epileptic children, migraineous and paralytic patients.  We have compared our results with normal healthy controls. We found that the bands ranges from 1151.94 to 1168.28 cm-1 due to phospholipids (P-O-C ) group appear in some of the migraineous and paralytic patients only. These bands are absent in normal and epileptic patients. The ...

Cholesterol gallstone disease is initiated in a liver which produces abnormal bile with excess cholesterol relative to bile salts and phospholipid. To define the responsible secretory mechanism(s), the rate of biliary lipid secretion was measured by a duodenal marker perfusion technique, while the bile salt pool was simultaneously estimated by isotope dilution. Two groups of control patients ex...