Mycelial cultures of Psilocybe cubensis capable of forming psilocybin and psilocin de novo display a high capacity for hydroxylation of tryptamine derivatives at the 4-position. A specific biotransformation of added synthetic N,N-diethyl-tryptamine was found. Thus high amounts of 4-hydroxy-N,N-diethyltryptamine (up to 3.3%) and a minor quantity of 4-phosphoryloxy-N,N-diethyltryptamine (0.01-0.8...

Mycelial cultures of PSILOCYBE CUBENSIS, with the ability to form psilocybin and psilocin DE-NOVO, also hydroxylated and methylated fed tryptamine to give psilocin in up to 3.3% dry mass of the obtained fruit bodies. By using HPLC and TLC, it was found that these mushrooms contain only a small amount of psilocybin (0.01-0.2% dry mass). The values of psilocin are the highest described in any mus...

Morphological and toxicological analyses were performed on hallucinogenic mushrooms that are currently circulated in Japan. Scanning electron microscope (SEM) indicated a three-dimensional microstructures in the mushrooms. The complementary use of SEM with an optical microscope was effective for observing characteristic tissues, such as basidiomycetes, spores, cystidia and basidia. Hallucinogen...

The biosynthesis of psilocybin has been investigated by feeding labelled precursors to Psilocybe cubensis. The following specifically labelled compounds were synthesized: psilocin-H, 4hydroxytryptamine-, N,N-dimethyltryptamine-C and --H, N-methyltryptamine-C-H and DLtryptophan-H. A number of other indoles were labelled by acid catalyzed exchange in tritiated water. The experimental data suggest...

Analysis of Psilocybe cubensis (Earle) Singer grown in controlled culture showed that the level of psilocin was generally zero in the first (or sometimes even the second) fruiting of the mushroom from a given culture and that the level reached a maximum by the fourth flush. The level of psilocybin, which was nearly always at least twice the level of psilocin, showed no upward or downward trend ...

We describe the use of high-fidelity single molecule sequencing to assemble genome psychoactive Psilocybe cubensis mushroom. The is 46.6Mb, 46% GC, and in 32 contigs with an N50 3.3Mb. BUSCO completeness scores are 97.6% 1.2% duplicates. Psilocybin synthesis cluster exists a 3.2Mb contig. dataset available from NCBI BioProject accessions <ns3:ext-link xmlns:ns4="...

Background and objectives: Considering the increasing prevalence of depression, many studies are launched to investigate new antidepressant treatments. The present research has shown how psilocybin as an active compound of Psilocybe cubensis (Earle) Singer extract (PCE) can change the parameters related to depression and anxiety in animal models. Both ...