There are several examples of heuristic rules first proposed from empirical observation, which eventually earned physicochemical validity after the fundamental derivation of a posteriori. This may be the case of Sherwood’s plot – for which a sound rationalization from first principles, entailing enthalpy and mass balances, is provided here; it applies to (fine) chemicals undergoing concentr...

The toxicity and corrosion potential of hydrogen sulfide in raw biogas underlines the need for biogas purification. Several techniques available for removal of hydrogen sulfide from biogas are out of the reach for common end users due to lack of knowledge, higher running costs, and insufficient operational skills. The present experimental study aims to propagate hydrogen sulfide removal techniq...

one of the most important aspects in recombinant biologic production, based on gmp rules, is the accuracy of final product quality control, especially assessment of host cell macromolecules contamination rate in final product. the purification requirement can be eliminated when the yeast cell containing the recombinant protein is used as a host cell. it is possibile that the final product conta...

background and objective: use of elastin-like proteins (elps) provides high-performance protein purification without need for chromatography. in line with cost reduction and facilitation of recombinant proteins purification, which represent a high percentage of production costs, in this project, we eliminated the need for proteases in the process of separation of recombinant proteins from elp b...

BACKGROUND Griffithsin is a broad spectrum antiviral lectin that inhibits viral entry and maturation processes through binding clusters of oligomannose glycans on viral envelope glycoproteins. An efficient, scaleable manufacturing process for griffithsin active pharmaceutical ingredient (API) is essential for particularly cost-sensitive products such as griffithsin -based topical microbicides f...

Dairy waste, more than any other trade waste has long since attracted the attention of specialists in the field of waste water purification. Many experiments have been made with artificial waste water, made from milk or milk products. Dairy wastes provide an excellent nutrient medium for many types of bacteria and fungi and, contrary to opinions held at one time, are very amenable to aerobic bi...

bacillus thuringiensis (bt) has been extensively used in biopesticidal formulations due to its safe environmental and human health records. the widespread use of bt is often challenged by production as well as formulation costs which are in direct contact with downstream processing, i.e. the separation/purification step. downstream separation/purification efficacy governs the marketability of a...

The economic performance of Sequential Hydrothermal Extraction (SeqHTE) and subcritical water extraction (SWE) was evaluated in this study for recovering bioactive compounds from potato peels. analysis included the cost estimation additional units required extract purification, such as polysaccharide separation, glycoalkaloid isolation, two final product purification alternatives: filtration or...

The intein-mediated purification system has the potential to significantly reduce the recovery costs of industrial recombinant proteins. The ability of inteins to catalyze a controllable peptide bond cleavage reaction can be used to separate a recombinant protein from its affinity tag during affinity purification. Inteins have been combined with a chitin-binding domain to serve as a self-cleavi...