Twelve populations of Escherichia coli B all lost D-ribose catabolic function during 2,000 generations of evolution in glucose minimal medium. We sought to identify the population genetic processes and molecular genetic events that caused these rapid and parallel losses. Seven independent Rbs(-) mutants were isolated, and their competitive fitnesses were measured relative to that of their Rbs(+...

Eight ruminally fistulated steers were utilized to evaluate the effects of amount of dried distillers grains (DDG) supplementation on forage dry matter intake (DMI), digestibility, and rumen parameters in steers consuming bermudagrass round bale silage (RBS). Treatments consisted of RBS only or RBS plus daily supplementation of DDG at 0.33, 0.66, or 1.0% of bodyweight (BW). There was quadratic ...

Induction of the chloramphenicol acetyltransferase gene cat-86 in Bacillus subtilis results from the activation of translation of cat-86 mRNA. The inducers, chloramphenicol and amicetin, are thought to enable ribosomes to destabilize a stem-loop structure in cat-86 mRNA that sequesters the ribosome binding site for the cat-86 coding sequence, designated RBS-3. The region of cat-86 mRNA which is...

DNA-DNA homology between a reduced bacteriophage sensitivity (Rbs+) probe and DNA from both Rbs+ and Rbs- Lactococcus lactis strains was examined. Homology was detected between the probe and five plasmids (pCI750, pCC34, pEB56, pNP2, and pJS88) isolated from lactose-positive Rbs+ transconjugants and between the probe and genomic DNA of a sucrose-positive Rbs+ transconjugant. Additionally, hybri...

Rutherford Backscattering Spectrometry (RBS) is a widely used method for the surface layer analysis of solids. This lecture gives a brief introduction into the method, and describes scattering kinematics, scattering cross-section data, stopping power data, detector resolution issues, and electronic energy loss straggling in some detail. Computer codes for the simulation of RBS spectra and RBS d...

Stimulated Raman backscattering (RBS) of intense laser radiation confined by a single-mode plasma channel with a radial variation of plasma frequency greater than a homogeneous-plasma RBS bandwidth is characterized by a strong transverse localization of resonantly-driven electron plasma waves (EPW). The EPW localization reduces the peak growth rate of RBS and increases the amplification bandwid...

Natural sleep aids are becoming more popular due to the widespread occurrence of sleep disorders. The objective of this study was to assess the sleep-promoting effects of rice bran-a product that is considered as a functional ingredient. To evaluate the sleep-promoting effects of a standardized rice bran supplement (RBS), we employed a pentobarbital-induced sleep test and conducted analyses of ...

The Moloney murine leukemia virus (MLV) repressor binding site (RBS) is a major determinant of restricted expression of MLV in undifferentiated mouse embryonic stem (ES) cells and mouse embryonal carcinoma (EC) lines. We show here that the RBS repressed expression when placed outside of its normal MLV genome context in a self-inactivating (SIN) lentiviral vector. In the lentiviral vector genome...

  The aim of this research was to evaluate the effects of oral recombinant bovine somatotropin (RBS) 1% on growth performance of rainbow trout (Oncorhynchus mykiss). 600 rainbow trout weighing 46±1 grams were randomly allotted in two groups including: 1) the control group and 2) RBS treated group, each in three replicates. The fish were hand-fed with commercial pellets. The fish received 200g R...

  The aim of this research was to evaluate the effects of oral recombinant bovine somatotropin (RBS) 1% on growth performance of rainbow trout (Oncorhynchus mykiss). 600 rainbow trout weighing 46±1 grams were randomly allotted in two groups including: 1) the control group and 2) RBS treated group, each in three replicates. The fish were hand-fed with commercial pellets. The fish received 200g R...