We present two new methods for purifying dinosterol (4a,23,24-trimethyl-5a-cholest-22E-en-3b-ol) from sediments for the purpose of hydrogen isotope analysis via gas chromatography–isotope ratio mass spectrometry (GC–IRMS). The first method uses reversed phase-high performance liquid chromatography (RP-HPLC) to purify dinosterol from structurally similar 4a-methyl sterols that co-elute on GC ana...

The isolation of a single DNA molecule for cloning is technically difficult, and the subsequent screening of colonies for recombinant DNA clones is time consuming. Ion pair-reversed phase HPLC (IP-RP-HPLC) analysis of nucleic acids improves the resolution and isolation of PCR products to be cloned. We demonstrate that PCR products analyzed and collected using the IP-RP-HPLC approach (WAVE DNA F...

the present study describes a simple, accurate, precise and cost effective uv-vis spectroscopic and rp-hplc method for the estimation of ibandronate sodium (ibn). the determination of ibandronate sodium (ibn) was performed by both uv and rp-hplc method using 215 nm as the determination wavelength. the drug was dissolved in naoh solution (0.1n naoh) for estimation in uv and in distilled water fo...

eperisone hydrochloride is a skeletal muscle relaxant and paracetamol is cyclooxygenase inhibitor. these drugs in combination are used as antispasmodics. a simple, specific, precise and accurate method was developed, namely reverse phase high performance liquid chromatography for simultaneous estimation of eperisone hydrochloride and paracetamol in tablet dosage form. in rp-hplc method separati...

oDays High-performance liquid chromatography (HPLC) for analysis of phospholipids has traditionally employed ultraviolet detection of the eluted compounds. The evaporative laser light-scattering detector (ELSD) offers new opportunities for quantitative analysis of lipids. Phospholipids were isolated from crude and degummed oils prepared from soyheans subjected to storage at high moisture conten...

The present study describes a simple, accurate, precise and cost effective UV-Vis Spectroscopic and RP-HPLC method for the estimation of Ibandronate sodium (IBN). The determination of Ibandronate sodium (IBN) was performed by both UV and RP-HPLC method using 215 nm as the determination wavelength. The drug was dissolved in NaOH solution (0.1N NaOH) for estimation in UV and in distilled water fo...

easily degradating and various isomeric forms of rapamycin (sirolimus) face the determination of this compound to many challenges. in this study, we developed and validated the isocratic reversed phase high performance liquid chromatographic (rp-hplc) method for rapamycin. separation was performed on a c8 column (mz, 15 × 4.6 mm, 5 µm particle size) using methanol:water (80:20 v/v) as the mobil...

oligosaccharide prepared from Pl.-B was eluted as a single peak on both reversed-phase {RP-) HPLC and size-fractionation (SF-) HPLC, and its strueture was estimated to be Manoct-qManal -. 3)(Xylfi1-.2) Manfi1-4GlcNAcfi1-4(Fuc=1-3)GlcNAc by a combination of component analysis, successive exogl)'cosidase digestions, IS-MS anal)'sis, and 500MHz 'H-NMR, Two tryptic gl)'copeptides were isolated from...