conventional methods for fungal identification in the clinical laboratory rely on morphological andphysiological tests. these tests often need several days or weeks to complete and are frequentlyunspecific. molecular identification mostly implies sequencing, which is relatively expensive andtime-consuming, as well as impractical for large numbers of isolates. the rolling circleamplification app...

conclusions species identification of trichophyton is crucially important for epidemiological and phylogenetic purposes and for genotype delineation. rca based on its polymorphisms can be used to generate identification barcodes and as an alternative to dna sequencing; it is a very fast, specific, and economical tool for species identification. results of the 61 putative trichophyton clinical i...

ABBREVIATIONS bp base pair cDNA complementary DNA CV coefficient of variation DNA deoxyribonucleic acid FRET fluorescence resonance energy transfer HRCA hyperbranched rolling circle amplification LN lymph node nt nucleotide OLA oligonucleotide ligation assay PCR polymerase chain reaction RCA rolling circle amplification RCR rolling circle replication RNA ribonucleic acid SCA serial circle ampli...

Cation-exchange-based chemiluminescence amplification (CXCLAmp) was developed in this study. After amplification by rolling circle amplification (RCA), the proposed RCA-CXCLAmp strategy was used to detect miRNA sensitively.

In molecular resolution refutation, the detection of empty clauses is important. We propose a rolling circle amplification-based detection method for resolution refutation. The rolling circle amplification (RCA) technique is known to be able to distinguish and amplify circular DNA. In this paper, we describe the representation of clauses and the RCA-based detection method. Bio-lab experiments s...

We report that QATPE, an aggregation-induced emission-active tetraphenylethene dye, can be used as a non-sequence-specific ssDNA probe for real-time monitoring of all rolling circle amplification (RCA) reactions, thus making RCA more suitable for biosensing applications.

Rolling Circle Amplification (RCA) is an isothermal enzymatic method generating single-stranded DNA products consisting of concatemers containing multiple copies of the reverse complement of the circular template precursor. Little is known on the compatibility of modified nucleoside triphosphates (dN*TPs) with RCA, which would enable the synthesis of long, fully modified ssDNA sequences. Here, ...

A novel rolling circle amplification (RCA) immunoassay based on DNA enriching magnetic nanoparticles and assembled fluorescent DNA nanotags, magnetic nanoparticles-RCA immunoassay, is developed as a versatile fluorescence assay platform for highly sensitive proteins detection.

A simple isothermal nucleic-acid amplification reaction, primer generation-rolling circle amplification (PG-RCA), was developed to detect specific nucleic-acid sequences of sample DNA. This amplification method is achievable at a constant temperature (e.g. 60 degrees C) simply by mixing circular single-stranded DNA probe, DNA polymerase and nicking enzyme. Unlike conventional nucleic-acid ampli...

Nucleic acid amplification is a hugely important technology for biology and medicine. While the polymerase chain reaction (PCR) has been highly useful and effective, its reliance on heating and cooling cycles places some constraints on its utility. For example, the heating step of PCR can destroy biological molecules under investigation and heat/cool cycles are not applicable in living systems....