Seedlings, rooted cuttings from juvenile stock plants, and cotyledon-derived tissue culture plantlets were propagated from several coastal Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) full-sib families so that the rooted cuttings and plantlets were clonally identical. The stock types (seedlings, rooted cuttings and plantlets) were planted in the field in spring 1987. In fall 1991, after f...

A protocol for rapid multiplication of Adhatoda vasica has been developed through nodal explants from field grown mature plants. The maximum number of shoots, i.e., 7.75 +/- 0.392 differentiated from split nodal halves on MS medium supplemented with BA (10.0 mg/l) during 4 weeks of culture. Maximum number of shoots formed per explant increased to ca. 30 within 6 weeks of subculture on medium co...

An in vitro regeneration protocol has been standardized via direct and indirect methods from excised root explants of C. bonduc, a threatened woody legume used for the treatment of contagious diseases, inflammation, leprosy, antiperiodic, febrifuge, anthelmenthic, urinary disorders, leucorrhoea, piles and to heal wounds. MS medium supplemented with 17.75 micromol BAP and 2.46 micromol IBA, indu...

Zygotic embryos excised from immature green fruits of the rattan palm, Calamus thwaitesii and cultured for 16 weeks under optimum culture conditions in Murashige and Skoog (MS) medium supplemented with 31.67 μM 2,4-dichlorophenoxyacetic acid (2,4-D) and 35.23 μM 2,4,5-trichlorophenoxyacetic acid (2,4,5-T) produced mixed (compact and friable) calli at 70 and 92 % rates. The semi-friable part of ...

Polyploid plantlets, including triploid, tetraploid, and mixoploid, were induced from the European pear (Pyrus communis L.) cultivar ‘Fertility’ by in vitro colchicine treatment of leaf explants. The leaf explants were incubated in 0.4% (w/v) colchicine for 24, 48, or 72 h, then transferred to adventitious shoot-induction medium. Regenerated shoots were pre-selected according to their morpholog...

An efficient plant regeneration protocol was developed from leaf and internode (stem) explants of Bacopa monniera L. on MS basal medium, supplemented with Cytokinins like BAP, KN, and TDZ used singly. The leaf cultures expressed maximum plant regeneration potential with 138.9 ± 0.50 shoots per explants on MS + 2.0 mg/ l BAP. The in vitro regenerated dwarf shoots were further elongated on MS med...

Background: Lemon verbena (Lippia citriodora H.B.K.) is an aromatic and medicinal plant of family Verbenaceae, which cultivated in the North region of Iran. Objective: This comparative study was carried out on essential oil content and composition of Lippia citriodora H.B.K. from micro-propagated and rooted apical stem cutting plantlets. Methods: The micro-propagated plantlets were obtained ...

Using cotyledons of 2-week old in vitro germinated seedlings, a rapid and efficient regeneration protocol was established for A. mangium on Murashige and Skoog (MS) basal medium. Cotyledons showed better direct shoot organogenesis producing maximum 10.88 ± 0.35 shoots per explant when cultured on MS medium supplemented with 2.0 μM BA and 1.0 μM NAA. Full strength of MS medium supplemented with ...

An efficient in vitro regeneration protocol was established for Swertia chirayita. The present study describes the regeneration of S. chirayita from shoot tip explants collected from wild grown plantlets. The highest number of shoots (26.10 ± 0.73) were obtained on Murashige and Skoog basal medium supplemented with 6-benzyladenine (BA) (0.5mg/l) in combination with gibberellic acid (GA3) (1.0 m...

background: lemon verbena (lippia citriodora h.b.k.) is an aromatic and medicinal plant of family verbenaceae, which cultivated in the north region of iran. objective: this comparative study was carried out on essential oil content and composition of lippia citriodora h.b.k. from micro-propagated and rooted apical stem cutting plantlets. methods: the micro-propagated plantlets were obtained by ...