Members of the saframycin/safracin/ecteinascidin family of peptide natural products are potent antitumor agents currently under clinical development. Saframycin MX1, from Myxococcus xanthus, is synthesized by a nonribosomal peptide synthetase, SafAB, and an O-methyltransferase, SafC, although other proteins are likely involved in the pathway. SafC was overexpressed in Escherichia coli, purified...

PURPOSE To describe and validate a semi-automated targeted sampling (SATS) method for quantifying optic nerve axons in a feline glaucoma model. METHODS Optic nerve cross sections were obtained from 15 cats, nine with mild to severe glaucoma and six with normal eyes. Optic nerves were dissected, fixed in paraformaldehyde and glutaraldehyde, and processed for light microscopy by resin embedding...

Classification is an important research area in cancer diagnosis. Fuzzy C-means (FCM) is one of the most widely used fuzzy clustering algorithms in real world applications. However there are two major limitations that exist in this method. The first is that a predefined number of clusters must be given in advance. The second is that the FCM technique can get stuck in sub-optimal solutions. In o...

Fourier Transform Infrared Spectroscopy (FTIR) is becoming a powerful tool for use in the study of biomedical conditions, including cancer diagnosis. As part of an ongoing programme of research into the potential early diagnosis of cervical cancer, Hierarchical Cluster Analysis (HCA) and Fuzzy C-Means (FCM) have been applied to distinguish FTIR spectra obtained from cancerous and non-cancerous ...

INTRODUCTION The aim of this study was to compare the shear bond strength of a self-adhering flowable composite (SAFC) and resin-modified glass ionomer (RMGI) to mineral trioxide aggregate (MTA) and calcium-enriched mixture (CEM) cement. METHODS AND MATERIALS A total of 72 acrylic blocks with a central hole (4 mm in diameter and 2 mm in depth) were prepared. The holes were filled with MTA (su...

Raw materials are a critical part of any cell culture medium; therefore, it is of utmost importance to understand and characterize them for high-quality product. The raw material characterization (RMC) program at SAFC focuses on individual screening of raw materials both analytically and biologically. The goal of the program is to develop the best-in-class knowledge base of the raw materials us...

The increasing complexity of present SoCs demands new, scalable, reusable, parallel interconnection models for their cores. This paper presents a comparison study made in an on chip reconfigurable multiprocessor, the X4CP32, on its interconnection. Three models were proposed, a bus system, a SoC using FIFO buffering, and a SoC using SAFC buffering. All the models were described in SystemC and s...

A membrane or an electrode binder to be used in a Solid Alkaline Fuel Cell (SAFC) needs to (i) be insoluble in both aqueous solutions and in the required fuels, and (ii) ha l-0 03 79 23 0, v er si on 1 28 A pr 2 00 9 Author manuscript, published in "J Polym Sci Part A: Polym Chem 47 (2009) 2043-2058" DOI : 10.1002/pola.23290

introduction: the aim of this study was to compare the shear bond strength of a self-adhering flowable composite (safc) and resin-modified glass ionomer (rmgi) to mineral trioxide aggregate (mta) and calcium-enriched mixture (cem) cement. methods and materials: a total of 72 acrylic blocks with a central hole (4 mm in diameter and 2 mm in depth) were prepared. the holes were filled with mta (su...