The biosorption performance of Phlomis cancellata Bunge as inexpensive, abundant and easily available adsorbent for safranin cationic dye removal from aqueous system has been evaluated in a batch process, using response surface methodology and employing a central composite design Experimental. The influence of different parameters such as, pH, contact time, agitation rate and sorbent dosage on ...

Histochemical reactions and radioautography were used to investigate the sequence of mast cell development in rat embryos. Mast cells arise ubiquitously in and are confined to the loose connective tissue in the embryo. The alcian blue-safranin reaction distinguishes between weakly sulfated and strongly sulfated mucopolysaccharides by a shift from alcian blue to safranin staining. Based on this ...

Moreira-Jacob (J. Bacteriol. 86:599, 1963) reported that 44 strains of Brucella suis were inhibited by safranin 0 in Brucella Agar (Albimi), whereas nearly all of 320 other strains of Brucella were not inhibited. Brinley-Morgan (World Health Organization, Brucellosis document , 246, 1963) examined the growth of more than 80 cultures on serum dextrose agar with added safranin 0, and found that a...

Magnetic micro-sized mesoporous silica particles were used for the preparation of a gated material able to release an entrapped cargo in the presence of an azo-reducing agent and, to some extent, at acidic pH. The magnetic mesoporous microparticles were loaded with safranin O and the external surface was functionalized with an azo derivative 1 (bearing a carbamate linkage) yielding solid S1. Aq...

Optical methods were tested for measuring the membrane potential changes of mitochondria in isolated perfused rat hearts. Safranin was found to be rapidly taken up by the Langendorff-perfused heart, and after loading with the dye there was practically no washout of the stain during perfusion with Krebs-Ringer bicarbonate solution. Staining with safranin induced the appearance of an intense abso...

Mitochondrial membrane potential (mtMP) is critical for maintaining the physiological function of the respiratory chain to generate ATP. The present study characterized the inter-relationship between mtMP, using safranin and tetramethyl rhodamine methyl ester (TMRM), and mitochondrial respiratory activity and established a protocol for functional analysis of mitochondrial bioenergetics in a mul...

BACKGROUND Proximal tubules subjected to hypoxia in vitro under conditions relevant to ischaemia in vivo develop an energetic deficit that is not corrected even after full reoxygenation. We have provided evidence that accumulation of nonesterified fatty acids (NEFA) is the primary reason for this energetic deficit. In this study, we have further investigated the mechanism for the NEFA-induced e...

The ability of sheep wool to remove Pb(II) ions and safranin dye from aqueous solutions through the adsorption process was investigated at room temperature. The metal ions concentration was determined by flame atomic absorption spectrophotometer method. The influence of pH, contact time, amount of adsorbent, temperature and initial metal...