The present study was conducted to determine the effect of seasonal and climatic variations on semen characteristics of Nili-Ravi buffalo breeding bulls in Sahiwal and Bahawalpur districts of Punjab, Pakistan. For this purpose, 14 adult NiliRavi buffalo breeding bulls (n=7 from each of Semen Production Unit (SPU) Qadirabad, Sahiwal and SPU Karaniwala, Bahawalpur), were used. Semen samples were ...

Naeem Akhtar MS Scholar; Department of Management Sciences, COMSATS Institute of Information Technology, Sahiwal, Pakistan Saqib Ali and Muhammad Salman MS Scholar; Department of Management Sciences, Bahauddin Zakariya University Sub Campus Sahiwal, Pakistan Asad-Ur-Rehman MS Scholar; Department of Management Sciences, COMSATS Institute of Information Technology, Sahiwal, Pakistan Aqsa Ijaz BBA...

Different semen assays were employed to determine the post -thaw fitness of buffalo and Sahiwal bull spermatozoa. Semen (two ejaculates) was collected from buffalo and Sahiwal bulls (six each) once a week for three consecutive weeks. Semen was diluted in lactose-fructose-egg yolk glycerol extender, equilibrated at 4 ac for 4 hr and frozen in liquid nitrogen vapors. A highly significant differen...

AIM To investigate the effect of temperature-humidity index (THI) on the expression pattern of heat-shock protein 72 (HSP72) mRNA of Sahiwal and Karan-Fries (KF) cattle in different THIs. MATERIALS AND METHODS Five different periods of a year were selected based on combinations of Tmax/Tmin, viz., P1: <20°C/<10°C; P2: >20°C/<10°C, P3: <30°C/<15°C; P4: >35°C/<20°C, and P5: >35°C/>20°C. The THI...

Present study was undertaken to compare the effect of two different milking methods (hand vs. machine milking) on milking ability in Murrah buffaloes and Sahiwal cows. 30 lactating Sahiwal cows and 30 lactating Murrah Buffaloes were selected and divided into four equal groups having 15 animals each. 15 animals of each species were subjected to machine milking and 15 animals of each species were...

Point mutations in exon IV of the bovine κ-casein (CSN3) gene determine two allelic variants, A and B. These variants were distinguished by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) analysis in the indigenous Sahiwal and Tharparkar cattle breeds. DNA samples (252 Sahiwal and 56 Tharparkar) were analyzed for allelic variants of the CSN3 gene. Polymorphism ...

Six apparently healthy, non-lactating and non-pregnant Sahiwal cows and Murrah buffalo each above two years of age were selected to evaluate the effect of seasonal variations on hematological parameters. Blood samples were collected aseptically by jugular vein puncture during peak winter and peak summer seasons. The present investigation revealed the mean values of Hb, PCV, TEC, neutrophils, MC...

The positional candidate gene DGAT1, within QTL of BTA14, harboring a lysine to alanine substitution (K232A) is significantly associated with milk production traits. The present investigation was undertaken to estimate the frequency of the DGAT1 K232A polymorphism in Indian zebu breed, Sahiwal (B. indicus; N= 51) and crossbred, Frieswal (HF X Sahiwal; N= 126) cattle using PCR-RFLP technique. De...

The major histocompatibility complex (MHC) plays well-defined roles in eliciting immune responses and combating infectious diseases. The major histocompatibility complex of cattle is referred to as BoLA (Bovine Lymphocyte Antigen). This genetic system is among the most polymorphic. In the present study, polymorphism of the BoLADRB3.2 locus in three Bos indicus breeds viz., Sahiwal, Rathi and Ha...

An epidemiological investigation was conducted on farms in Tororo and Soroti districts of Uganda from January to February 2000 to determine the cause of reported persistent mortality of cattle. Blood and faecal material of 98 cattle comprising of 33 Friesians, 58 Zebu and 7 Sahiwal were examined. Results revealed that seven (7.1 %) cattle had trypanosome infection, mainly due to Trypanosoma viv...