dna sequence determination is a tremendous human achievement. dna sequencing includes several methods and technologies in use for determining the order of the nucleotide bases (adenine, guanine, cytosine, and thymine) in a molecule of dna. knowledge of dna sequences has become indispensable for basic biological research, other research branches utilizing dna sequencing, and in numerous applied ...

genetic typing methods of t. gondii strains have been extensively perfected in recent years. from a technical point of view, many tools usable for genetic studied on single-copy loci have been used: rflp, pcr-rflp, sequencing, rapd-pcr and isoenzyme analysis. we described the cloning and sequence analysis of the gene which encodes the major surface antigen (sag1 or p30) of t. gondii. sag1 is th...

tarkhineh is one of the traditional foods in iran and a rich source of probiotic bacteria. the objective of this study was to identify the probiotic bacteria isolated from tarkhineh using 16s rrna gene sequencing and molecular typing with repetitive extragenic palindromic–pcr (rep-pcr). in total, 20 different bacteria were isolated from traditional dairy products and tarkhineh. molecular identi...

conclusions several previous studies used pcr-rflp to genotype hbv; however, we showed the high risk to obtain atypical profiles, especially in advanced stages of chronic infection, with as results difficulties to genotype the virus. these profiles resulted from the accumulation of mutations during natural course of infection resulting in a modification in restriction sites for enzymes. so, we ...

context: detection of causative pathogen is crucial for treatment strategies in most infections. molecular diagnosis as a more sensitive and specific method, has become the best option to investigate certain pathogens, mainly viruses. recent scientific and technological approaches resulted in changing the area of diagnostic microbiology. evidence acquisition: a comprehensive online search of da...

objective(s): breast cancer is the most common cancer in women. every year, one million new cases are reported worldwide, representing 18% of the total number of cancer in women. hereditary brca1 and brca2 mutations account for about 60% of inherited breast cancer and are the only known causes of hereditary breast cancer syndrome. the aim of this study was to determine the frequency of brca2 (e...

background and objectives : dna molecular weight marker is widely used in molecular biology experiments incurring considerable costs on low-budget settings. materials and methods : here a pcr-supported procedure is described that uses 10 primer pairs targeting chromosomal dna from the harmless vaccinal bacillus anthracis sterne 34f2 strain as template. a single pcr protocol is used to reproduce...

leptospirosis caused by infection with pathogenic leptospires, which is the most prevalent zoonotic disease in the world. the outer membrane proteins (omps) of pathogenic leptospires such as lipl41 play a crucial role in pathogenesis of this disease. therefore a major challenge to develop an effective vaccine against leptospirosis is application of basic research on the omps of leptospires to i...

given the importance of transcriptome analysis in various biological studies and considering thevast amount of whole transcriptome sequencing data, it seems necessary to develop analgorithm to assemble transcriptome data. in this study we propose an algorithm fortranscriptome assembly in the absence of a reference genome. first, the contiguous sequencesare generated using de bruijn graph with d...

Technology has been playing a very important role in the advancement of science, and big breakthroughs science are dependent upon technologies available from time to time. Between technology, good understanding is required so that can be used most efficiently effectively solving targeted questions scientific research. Genome nucleotide sequencing as an technology after several generations techn...