interferon is a protein secreted by eucaryotic cells following stimulation by viruses, bacteria, and many other immunogenes. recent medical studies indicate that interferons have effective role in the treatment of virus infections, immunodeficiency and certain types of cancer such as hairy cell leukaemia (hcl). the aim of the present study is to apply yeast strain for secreting human ifnα2b fol...

Interferon is a protein secreted by eucaryotic cells following stimulation by viruses, bacteria, and many other immunogenes. Recent medical studies indicate that interferons have effective role in the treatment of virus infections, immunodeficiency and certain types of cancer such as hairy cell leukaemia (HCL). The aim of the present study is to apply yeast strain for secreting human IFNα2b fol...

background: eukaryotic proteins generally have signal peptides, which are not only crucial for their secretion efficiencies but are important for their expression levels. the coagulation factor ix (fix) is a glycoprotein that plays a fundamental role in a blood coagulation pathway. reduced levels or dysfunctional fix are associated with hemophilia b. to improve the hfix secretion efficiency in ...

background: in the recent years, there has been an increasing interest in secretory production of recombinant proteins, due to its various advantages compared with intracellular expression. signal peptides play a critical role in prosperous secretion of recombinant proteins. accordingly, different signal peptides have been assessed for their ability to produce secretory proteins by trial-and-er...

cloning and sequencing of a lipase gene from an indigenous bacillus pumilus, strain f3, revealed an open-reading frame of 648 nucleotides predicted to encode a protein of 215 residues. sequence analysis showed that f3 lipase contained a signal peptide composed of 34 amino acids with an h domain of 18 residues. a tat-like motif was found in the signal peptide similar to some other bacillus pumil...

Cloning and sequencing of a lipase gene from an indigenous Bacillus pumilus, strain F3, revealed an open-reading frame of 648 nucleotides predicted to encode a protein of 215 residues. Sequence analysis showed that F3 lipase contained a signal peptide composed of 34 amino acids with an H domain of 18 residues. A tat-like motif was found in the signal peptide similar to some other Bacillus pumil...

In this study, the cDNA Growth Hormone (cGH) of the Belugasturgeon (Husohuso) and Russian sturgeon (Acipensergueldenstaedtii) were cloned and sequenced, and phylogenetic relationships were examined using nucleic acid and amino acid sequences. The nucleotide sequence of the Beluga GH has an open reading frame of 645 nucleotides encoding a protein 214 amino acid residues. The signal peptide cleav...

in this study, the cdna growth hormone (cgh) of the belugasturgeon (husohuso) and russian sturgeon (acipensergueldenstaedtii) were cloned and sequenced, and phylogenetic relationships were examined using nucleic acid and amino acid sequences. the nucleotide sequence of the beluga gh has an open reading frame of 645 nucleotides encoding a protein 214 amino acid residues. the signal peptide cleav...

conclusions the results of this study demonstrated that the tpasp-padre-truncated orf2 gene cassette and the truncated orf2 gene in recombinant plasmids are successfully expressed in eukaryotic cells. the immunogenicity of the two recombinant plasmids with different formulations will be evaluated as a novel dna vaccine in future investigations. results sequence analysis and bioinformatics studi...